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Aiming at the current reality of low accuracy of fertilizer regulation and control during the fertilizer application operation of oilseed rape direct seeding machine, and the difficulty of realizing spatiotemporal differentiated fertilizer replenishment with different soil fertility distributions, an accurate variable fertilizer application linear self-resistant regulation and control system was designed-based on the prescription map for oilseed rape. A method of raster division of the fertilizer prescription map-based on the cloud-based high-precision map planning operation path was proposed, which provided a guiding fertilizer amount for the machine fertilizer application. A linear self-immunity control algorithm for fertilizer dischargers was constructed. Matlab simulation determined the key parameters, and comparison with the PID control algorithm showed that the linear self-immunity control algorithm improved the anti-interference, overshooting amount, and steady-state error by 39.08%, 91.77%, and 86.96%, respectively, compared with the latter. The results of the bench test showed that the average control accuracy of the fertilizer dispenser’s granular fertilizer discharge was 98.06% under the set fertilizer application rate and operating speed of the fertiliser application regulation system. The road test results indicated that the prescription map-based variable fertilization control system, after compensating for lag distance during variable fertilization operations, achieved an average fertilization position lag distance of 0.28 m, with the control accuracy of fertilizer application volume exceeding 95.67% across different positions. The results of the field test showed that the linear self-immunity control algorithm had a control accuracy of no less than 95.21% of the fertilizer amount in different grid areas, which was better than the PID control algorithm. The research result can provide an effective reference for accurate variable fertilizer application-based on soil nutrients in oilseed rape production.