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In order to explore the fertilization mode of emission reduction, superior yield and high efficiency, taking winter wheat (Xiaoyan 22) as the research object, the experiment set six treatments: no fertilization ( KB ), conventional fertilization ( NP ), and organic fertilizer substituting 25% (25% OF),50% (50% OF), 75% (75% OF), and 100% (100% OF) of inorganic fertilizer on equal nitrogen basis, was used to analyze the effects of different proportions of combined organic and inorganic fertilizer application on greenhouse gas emissions, yield, and nitrogen use efficiency in winter wheat farmland, put forward the combined organic-inorganic fertilization mode with the goal of emission reduction, superior yield and high efficiency. The results showed that in the two winter wheat growing seasons, the average cumulative CO2 emissions of 75% OF and 25% OF were the lowest, which was decreased by 7.62% and 15.31% compared with that of NP, respectively. The CO2 emission flux was increased in summer and decreased in winter. Among all fertilization treatments, NP had the highest CH4 absorption, while 100% OF had the lowest CH4 absorption. The absorption of CH4 in soil was decreased with the increase of the amount of organic fertilizer. The cumulative N2 O emissions of 75% OF and 100% OF were the lowest, which was decreased by 92.94% and 159.47% compared with that of NP, respectively. The global warming potential (GWP) and greenhouse gas intensity (GHGI) of 75% OF were the lowest, which was decreased by 27.19% and 41.38% compared with NP, respectively. Compared with NP, the winter wheat yield of 50% OF and 75% OF was increased by 15.78% ~ 17.73% and 18.64% ~ 23.07% , respectively, from 2018 to 2020. Compared with conventional fertilization, 75% OF significantly increased the nitrogen uptake and nitrogen utilization efficiency of winter wheat. Considering the economic and ecological factors, replacing 75% inorganic fertilizer with organic fertilizer can take into account the requirements of emission reduction, superior yield and high efficiency, and provide a reference for the better fertilization mode of winter wheat in Northwest China.