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With the rapid development of agricultural and rural economy, the problem of rural labor shortage has become increasingly prominent. As one of the important links, picking has been developed to develop a riding tea picking machine to improve the mechanization level of tea gardens.Based on the principle of human biomechanics, the effects of human body parameters on vertical dynamic equivalent mass, horizontal dynamic equivalent mass and seat-head transfer function were analyzed by establishing a human body model consistent with human vibration characteristics.Through the simulation of vertical transfer rate, rotational transfer rate and apparent mass, it was concluded that low-frequency resonance occurred in the range of 4 ~6 Hz,，8 ~10 Hz and 20 ~30 Hz.At the same time, tea field test was carried out on the whole vehicle, and objective data such as driver vibration acceleration was obtained by acceleration sensor, and the transmission, time domain and frequency domain characteristics in the resonant frequency range of high frequency and low frequency band were analyzed. Then by installing polyurethane foam seats and other vibration reduction means to reduce the transmission rate and avoid resonance, the purpose of improving the comfort of the riding tea picker was achieved. The test results showed that in the low frequency band，the transmission rate at the seat was reduced from 0~1.0 to 0~0.2, and the peak value was reduced by 80%, thus improving the noise and vibration performance of the riding tea picker. The human body model had a certain prediction accuracy, which can provide an important reference for human machine interface design in dynamic environment, and has a certain guiding significance for comfort in vibration environment and further research on human vibration.