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不同微藻培養(yǎng)處理下餐廚垃圾沼液氨吹脫剩余液凈化效果研究
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金華市科技計劃項目(2022-3-066)和農(nóng)業(yè)農(nóng)村部種養(yǎng)結合重點實驗室開放項目(202306)


Purification Effects of Ammonia Stripping Residual Liquid from Anaerobic Digestion Food Effluent by Two Microalgae Cultivation
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    摘要:

    餐廚垃圾沼液富含豐富的營養(yǎng)物質,,沼液經(jīng)過氨氮吹脫后的剩余液仍然含有大量的氮磷養(yǎng)分,,是微藻培養(yǎng)潛在的優(yōu)質培養(yǎng)基。選取小球藻和四尾柵藻兩種微藻為研究對象,,對餐廚垃圾沼液氨吹脫后的剩余液(本文中稱為“氨吹脫沼液”)進行微藻培養(yǎng)處理,,研究其在不同濃度沼液 (質量分數(shù)20%、40%,、60%,、80%和100%) 中的生長特性、對沼液中污染物凈化效果以及胞外聚合物的分泌情況,。研究結果顯示,,兩種微藻在中低濃度沼液(質量分數(shù)40%~60%)中生長狀態(tài)良好,小球藻在質量分數(shù) 40% 的沼液中可獲得最高生物量 1.0 g/L,,四尾柵藻在沼液質量分數(shù)為60%時獲得最大生物量0.9 g/L,,但是在高濃度沼液(質量分數(shù)80%~100%)中微藻生長受到一定抑制,氮磷的去除效果降低,。利用中低濃度沼液(質量分數(shù)20%~60%)培養(yǎng)小球藻和四尾柵藻時,,對總氮、硝態(tài)氮,、總磷和化學需氧量的去除效果較好,,小球藻對應的去除率最高分別為41.14%,、48.64%、77.70% 和 62.08%;四尾柵藻對應的去除率最高分別為59.10%,、58.39%,、82.65%和63.43%,高于小球藻,。經(jīng)過胞外聚合物分析,,發(fā)現(xiàn)隨著培養(yǎng)時間的延長,微藻的胞外聚合物含量先增加后減少;小球藻在40%沼液中含量最高,,而四尾柵藻在80%沼液中含量最高,,且通過三維熒光光譜分析發(fā)現(xiàn),胞外聚合物主要有機物為溶解性微生物副產(chǎn)物和富里酸,。因此,,綜合考慮微藻生物量、氮磷養(yǎng)分去除效率和沼液稀釋成本,,四尾柵藻在餐廚垃圾沼液處理中表現(xiàn)出較高的適應性,。

    Abstract:

    Anaerobic digestion food effluent (ADFE) is rich in nutrients, and the residual liquid after ammonia stripping still contains a large amount of nitrogen and phosphorus nutrients, making it a potential high-quality culture medium for microalgae cultivation. Two microalgae,Chlorella sp. and Scenedesmus quadricauda(S. quadricauda), were selected as the objects of study, and the residual liquid after ammonia stripping of ADFE (referred to as “ammonia stripping digestate”) was cultured,,and the growth characteristics, purification effect of pollutants and secretion of extracellular polymers (EPS) were investigated in the digestate at different concentrations (20%,,40%,60%,,80% and 100%). The results showed that both microalgae species grew well in medium to low concentrations of digestate (40%~60%). Chlorella sp. achieved the highest biomass of 1.0 g/L in 40% concentration of ammonia stripped digestate, while S. quadricauda obtained the maximum biomass of 0.9 g/L at a 60% concentration. However, in high concentrations of digestate (80%~100%), the growth of microalgae was somewhat inhibited, and the removal efficiency of nitrogen and phosphorus was decreased. When cultivating Chlorella sp. and S. quadricauda with medium to low concentrations of digestate (20%~ 60%), the removal effects on total nitrogen, nitrate nitrogen, total phosphorus, and chemical oxygen demand (COD) were the best. The highest removal rates for Chlorella sp. were 41.14%,,48.64%,77.70%, and 62.08%, respectively;while for S. quadricauda, the highest removal rates were 59.10%,,58.39%,,82.65%, and 63.43%, which were higher than those of Chlorella sp. Analysis of EPS also revealed that the content of EPS in microalgae was firstly increased and then decreased with extended cultivation time. Chlorella sp. had the highest content in 40% digestate, whereas S.quadricauda had the highest content in 80% digestate. Furthermore, three-dimensional fluorescence spectroscopy analysis indicated that the main organic components of EPS were soluble microbial byproducts and fulvic acids. Therefore, considering the microalgal biomass, nitrogen and phosphorus nutrient removal efficiencies, and the cost of digestate dilution,S. quadricauda showed high adaptability in ADFE treatment. The research result can provide insights into the sustainable management and resource utilization of digestate.

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郭春春,郭亭,王亮才,徐劍翔,董仁杰,柳珊.不同微藻培養(yǎng)處理下餐廚垃圾沼液氨吹脫剩余液凈化效果研究[J].農(nóng)業(yè)機械學報,2024,55(s2):330-339. GUO Chunchun, GUO Ting, WANG Liangcai, XU Jianxiang, DONG Renjie, LIU Shan. Purification Effects of Ammonia Stripping Residual Liquid from Anaerobic Digestion Food Effluent by Two Microalgae Cultivation[J]. Transactions of the Chinese Society for Agricultural Machinery,2024,55(s2):330-339.

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  • 收稿日期:2024-08-08
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  • 在線發(fā)布日期: 2024-12-10
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