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基于特征波長的接觸式作物葉綠素檢測系統(tǒng)
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國家自然科學(xué)基金項(xiàng)目(32371995)、山東省重點(diǎn)研發(fā)計(jì)劃(重大科技創(chuàng)新工程)項(xiàng)目(2022CXGC020708-1)和中國農(nóng)業(yè)大學(xué)研究生教改項(xiàng)目(JG202026,、QYJC202101,、JG202102、BH2022176)


Contact-based Crop Chlorophyll Detection System Based on Feature Wavelengths
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    摘要:

    基于葉綠素分子在可見光和近紅外光譜區(qū)間(波段400~1 000 nm)對光的吸收和反射的敏感特性,,設(shè)計(jì)了一種接觸式作物葉綠素檢測系統(tǒng),,來實(shí)現(xiàn)作物葉綠素含量的無損、快速和準(zhǔn)確檢測,。使用高光譜相機(jī)采集玉米葉部397~1003 nm反射光譜,,并使用分光光度法萃取葉片葉綠素含量真值,開展葉綠素敏感響應(yīng)波長篩選,。經(jīng)蒙特卡洛無信息變量消除(MCUVE)算法在10~50個特征波長內(nèi)進(jìn)行變量篩選,,發(fā)現(xiàn)采用30個特征波長時具有最優(yōu)的葉綠素含量檢測能力,同時通過連續(xù)投影(SPA)算法進(jìn)行特征波長篩選,,2種算法共得到7個重合特征波長,,又通過對波段和葉綠素含量進(jìn)行相關(guān)性分析,剔除低相關(guān)性波段,,最終得到6個特征波長,。根據(jù)篩選出的特征波長對接觸式圖像傳感器波段進(jìn)行選型,設(shè)備的硬件主要包括傳感器圖像采集,、主控制器,、電源等模塊,實(shí)現(xiàn)作物葉部近紅外和可見光反射光譜數(shù)據(jù)采集,、處理、顯示和存儲功能,。開展傳感器性能測試和田間應(yīng)用測試,,通過分析獲取的多光譜圖像的反射率構(gòu)建葉綠素含量偏最小二乘(PLS)檢測模型,驗(yàn)證集決定系數(shù)為 0.705;通過分析各植被指數(shù)與葉綠素含量的相關(guān)性,,選取了相關(guān)性較高的歸一化紅邊植被指數(shù)(NDRE),、綠紅差值植被指數(shù)(GMR)和地面葉綠素指數(shù)(MTCI)進(jìn)一步組合建模,檢測模型精度提高到0.713,,最終將模型嵌入系統(tǒng)實(shí)現(xiàn)了田間葉綠素含量快速檢測,,為作物長勢分析提供了技術(shù)支持,。

    Abstract:

    Based on the sensitive characteristics of chlorophyll molecules to light absorption and reflection in the visible and near-infrared spectral range (400~1 000 nm), a crop chlorophyll detector based on a contact image sensor can be designed to achieve non-destructive, rapid, and accurate detection of crop chlorophyll content. Firstly, a hyperspectral camera was used to collect the reflection spectrum of cornleaves in the range of 397~1003 nm, and the true value of leaf chlorophyll content was extracted by using spectrophotometry. Nextly, the screening of chlorophyll - sensitive response wavelengths was carried out. The Monte Carlo uninformative variable elimination(MC - UVE)algorithm was used to screen variables within the range of 10 to 50 feature wavelengths, and it was found that using 30 feature wavelengths provided the optimal detection capability for chlorophyll content. Simultaneously, the successive projections algorithm(SPA)was employed for feature wavelength screening. The two algorithms yielded a total of seven overlapping feature wavelengths. Further, through correlation analysis between the bands and chlorophyll content, low-correlation band was eliminated, ultimately resulting in six feature wavelengths. The selected feature wavelengths were used to choose the bands for the contact image sensor. The hardware of the device mainly included sensor image acquisition, main controller, display, and other modules, which realized the functions of near - infrared and visible light reflection spectrum data acquisition, processing, display, and storage of crop leaves. Sensor performance tests and field application tests were conducted. By analyzing the reflectivity of the obtained multispectral images, a partial least squares detection model for chlorophyll content was constructed,with a coefficient of determination for the validation set of 0.697. By analyzing the correlation between various vegetation indices and chlorophyll content, the normalized difference red edge(NDRE), green minus red(GMR), and normalized difference red edge(MTCI)vegetation indices with higher correlation were selected for further combined modeling, improving the detection model accuracy to 0.706. The model was embedded into the system, ultimately achieving rapid detection of chlorophyll content in the field and providing technical support for crop growth analysis.

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張春琪,劉夢姝,晁金陽,唐彬,李民贊,孫紅.基于特征波長的接觸式作物葉綠素檢測系統(tǒng)[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2024,55(s2):255-262. ZHANG Chunqi, LIU Mengshu, CHAO Jinyang, TANG Bin, LI Minzan, SUN Hong. Contact-based Crop Chlorophyll Detection System Based on Feature Wavelengths[J]. Transactions of the Chinese Society for Agricultural Machinery,2024,55(s2):255-262.

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  • 收稿日期:2024-07-28
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  • 在線發(fā)布日期: 2024-12-10
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