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The long-term traditional farming methods in the southwest region have caused the plough bottom to move up and the soil to become increasingly barren. The traditional subsoiling and fertilization devices have poor operating efficiency. Combined the principles of bionics, a layered subsoiling and classified fertilization device was designed, a discrete element model of soil and fertilizer was established based on EDEM software, and simulation experiments were conducted. The simulation results showed that the optimal parameter combination for the subsoiling device was a subsoiling speed of 1.54 km/h, a horizontal spacing of 500 mm between the front and rear shovels，and a vertical spacing of 300 mm between the front and rear shovels;The optimal parameter combination for the fertilizer feeder device is a radius of 30 mm for the sheave, a distance of 28.5mm from the arc center, and a helix angle of 81°. Based on the simulation results, field experiments were conducted, and the results showed that the disturbance rate of the layered subsoiling and classified fertilization machine on the soil was 52.4%, the soil looseness was 19.7%, and the soil crushing rate was 78%, all meeting the design requirements of the subsoiler and surpassing the single-shovel subsoiling operation. The results of the fertilizer performance test showed that the organic fertilizer application rates were 120.8 and 182.8 g, respectively, while the inorganic fertilizer application rates were 620.3 and 916.9 g, respectively. The stable variation coefficients of organic fertilizer and inorganic fertilizer were 2.38% and 4.93%, respectively, meeting the requirements for fertilizer application and achieving the stratified application of organic and inorganic fertilizers. The integrated device for layered subsoiling and classified fertilization designed improved the soil crushing effect and fertilization efficiency of the plough bottom layer, providing a reference for optimizing the structure of conservation tillage machinery.