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Although existing sugarcane leaf strippers can effectively remove most sugarcane leaves, they mainly focus on the sugarcane for sugar extraction, without considering the damage to sugarcane bud, so they are unsuitable for sugarcane seed. To solve the problem of high bud damage rate in sugarcane leaf strippers, a whole-stem seed leaf stripping system was designed to conduct simulation analysis and experimental research. Firstly, a single-factor simulation experiment was performed on the whole-stem sugarcane seed stripping process by a virtual simulation platform to analyze the influences of the crushing mechanism, feeding and discharging mechanism, and stripping mechanism on the sugarcane seeds. Secondly, based on simulation analysis, a physical testing platform experiment was completed. According to the simulated experimental parameters, the quadratic regression universal rotation combination experiment was performed, and parameters were optimized. Finally, the optimal relevant operating parameters of the sugarcane seed stripping system were obtained: the flexible-rope-crushing-roller speed was 670r/min, the stripping-roller speed was 400r/min, the single row number of flexible rope crushing elements was 16, and the length of the flexible rope crushing elements was 330mm, the trash content of sugarcane seed stripping was 6.59%, and the sprout damage rate was 4.70%. Finally, a comparative experiment was performed between the sugarcane seeds stripped by the optimal parameters and those stripped by traditional leaf stripping machines. The experimental results showed that the germination rate of sugarcane seeds planted under the whole-stem sugarcane seed stripping system was 81.25%, which was higher than the 56.25% germination rate of sugarcane seeds stripped by traditional leaf stripping machines. These can effectively improve the survival rate of sugarcane sprouts and have practical significance and application prospects for the development of sugarcane.