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The research on the trade-off synergy relationship of multifunctional cultivated land is of great significance for protecting cultivated land resources, ensuring food security, maintaining ecological security, and promoting rural revitalization. Taking 105 counties in Henan Province as the research object, and methods such as Spearman rank correlation analysis and K-means clustering analysis were used to study the multifunctional level of cultivated land in Henan Province from 2000 to 2020 and its trade-off and synergy relationship. The results showed that from 2000 to 2020, the multifunctional index of cultivated land in Henan Province showed an overall upward trend, showing a spatial pattern of “high in the east and low in the west”, and different spatiotemporal differentiation characteristics were observed among various functions. The grain production function in the eastern region was strong, while the ecological service function in the western region was excellent. The overall economic contribution function was declining, and although the social security function was weak, it was gradually improving. The spatiotemporal differences in the trade-off synergy relationship of multifunctional cultivated land were significant, and the interactions between multiple functions showed a characteristic of weakened synergy and intensified trade-offs, especially the significant trade-off between food production and economic contribution functions, as well as ecological service functions. Based on the dominant functional types of cultivated land and the characteristics of multifunctional coupling coordination, Henan Province was divided into four types of functional zones: modern agricultural demonstration zone, urban agricultural leisure zone, ecological agricultural construction zone, and modern agricultural construction zone, and differentiated control measures were proposed. The research result had certain theoretical and practical significance for guiding the rational allocation of cultivated land in major grain producing areas and improving the efficiency of diversified utilization of cultivated land.