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In the process of seedling raising in the greenhouse hole tray, about 5% to 20% of the holes had the phenomenon of missed seeding, stunted development, etc. In order to improve the utilization rate of the hole tray and ensure the quality of the finished seedlings, it was necessary to remove the inferior seedling substrate block neatly and replenish the healthy bowl seedlings in time. There were two common rejection ends: finger shovel type and air suction type. There were problems such as the finger shovel type seedling removal method and the air suction seedling removal method. The finger shovel type seedling was easy to cause the bowl body to be loose, fragile and difficult to grasp and the air suction seedling removal method had a low rejection rate under high moisture content. The conveyor belt was used to move the hole plate, and the lifting module drove the traversing module to lift, and two seedling removal end effector were installed on the traversing module, and the culling end-effector used spiral blades to break up the substrate. The matrix in the hole was broken and transported to the top soil storage tank by means of spiral conveying and negative pressure adsorption, and automatically transferred it to the soil discharge tank when it was lifted. Taking the 200-hole strip matrix cavity disc as the research object, the soil lifting mode of the spiral blade was theoretically analyzed and the relevant design parameters were determined, and the path planning of the spiral inferior seedling rejection device was carried out. The test platform of the spiral inferior seedling rejection device was built and the four-factor and three-level orthogonal experimental research on the removal of spiral substrate was carried out. The influence of experimental factors, from large to small, included notch cutting angle, pitch, rotation speed and lifting speed. The optimal parameters were determined to be 16 mm pitch, 10° notch cutting angle, 2 500 r/min rotation speed and 10 mm/s lifting speed. The performance verification test was carried out under the optimal combination of parameters, and the results showed that the average operating efficiency of the whole machine was 832 holes/h (42 trays/h). The average rejection rate of the substrate was over 83%, which met the needs of the seedling rejection operation.