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In order to investigate the influence of different positions of apple tree branches on the picking effect under different combinations of vibration parameters, the main factors affecting the apple drop were obtained as vibration frequency, amplitude and holding position through the establishment of a secondary branch vibration mechanics model of the apple tree and analytical analyses. Measuring the morphological characteristics of apple trees and based on the principle of dwarf anvil dense planting shaping to establish a three-dimensional model of fusiform apple trees, using ANSYS software to carry out finite element simulation analysis of the apple tree model, the simulation results showed that the vibration frequency of 4~8Hz,amplitude of 20~30mm, the holding position of 0.35l~0.65l (l was the length of secondary branches), the damage to the fruit tree was small and easy to fall off the apples. In order to determine the best combination of vibration parameters for different positions of branches in apple trees, a four-factor, three-level field experiment was designed, and the experimental data were analyzed and response surface was optimized by using Design-Expert software, and the results of the parameter optimization were as follows: when picking apples on the upper layer of the apple tree, the vibration frequency was 5Hz, the amplitude was 28mm, and the clamping position was 0.40l. When picking apples in the middle layer of the apple tree, the vibration frequency was 4Hz, the amplitude was 30mm, and the clamping position was 0.43l; when picking apples from the lower layer of the apple tree, the vibration frequency was 8Hz, the amplitude was 20mm, and the clamping position was 0.65l; it can be seen through the validation test that the picking rates of the upper to the lower layer of the apple tree were 96.4%, 94.8%, and 93.2%, which were similar to the optimized values, indicating that the optimized model was reliable.