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To explore the applicability of the DNDC model in simulating N2O emission from red soil slope farmland, two tillage treatments (conventional tillage and no-tillage) were set up for peanut field in northern Jiangxi Province. Field in-situ observation experiments of N2O emission were carried out for three consecutive years (2019—2021) by static box-gas chromatography to study the characteristics of N2O emission and the simulation effect of DNDC model under different tillage treatments. The results showed that the DNDC model had a good simulation effect on soil temperature in 0~10cm (the correlation coefficient r was 0.86~0.87) and crop yield (r was 0.90) under different tillage treatments. The model could effectively simulate the fluctuation of N2O emission which caused by fertilization and rainfall during peanut season, as well as the peak of N2O emission under conventional tillage. However, this model would underestimate the N2O emission peak and its total amount to some extent under no-tillage, and had a smaller response to rainfall which was below 16mm. Soil pH value and fertilization amount had the greatest impact on N2O emissions from sloped peanut field on red soil. Rainfall, soil organic carbon and clay content were also important factors affecting N2O emissions. According to simulate N2O emission and peanut yield under different amount of fertilizer application in 2019, it was found that the application amount of nitrogen fertilizer should not be less than 76.54kg/hm2, and should not exceed 106.78kg/hm2. The research results can provide theoretical basis for optimizing crop planting and reducing agricultural greenhouse gas emission in red soil slope farmland.