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便攜式土壤土霉素提取-檢測裝置設(shè)計(jì)與試驗(yàn)
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江蘇高校優(yōu)勢學(xué)科建設(shè)工程(三期)項(xiàng)目(PAPD-2018-87),、中國博士后科學(xué)基金項(xiàng)目(2022M713499)和江蘇大學(xué)高級人才科研啟動基金項(xiàng)目(JDG19026)


Design and Experiment on Portable Device for Extraction and Detection of Oxytetrarycline in Soil
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    摘要:

    土壤中土霉素(Oxytetracycline,,OTC)高性能的現(xiàn)場分析方法對于保護(hù)生態(tài)環(huán)境安全和維護(hù)人類健康具有重要意義。針對土壤中痕量OTC的現(xiàn)場檢測難題,,設(shè)計(jì)功能集成的便攜式提取-檢測裝置,,用于土壤中OTC的現(xiàn)場提取與精準(zhǔn)分析。首先,,基于集成電路技術(shù)與3D建模,,研制具有稱量、攪拌與離心功能的便攜式裝置,,并通過與實(shí)驗(yàn)室用提取裝置的性能對比,,驗(yàn)證裝置的提取精度;分別以LED和便攜式電化學(xué)工作站為光電化學(xué)檢測的光源驅(qū)動和信號采集裝置,,研制便攜式檢測裝置;進(jìn)行土壤中OTC的現(xiàn)場分析試驗(yàn),。結(jié)果表明,,研制的便攜式裝置對土壤OTC的提取精度較高,檢測的線性范圍為1×10-8~1×10-6mol/L,,檢出限為5.33×10-9mol/L,;在現(xiàn)場分析試驗(yàn)中,對土壤中OTC檢測的加標(biāo)回收率為92%~97%,,相對標(biāo)準(zhǔn)偏差為1.8%~5.2%,,且準(zhǔn)確度得到了國標(biāo)法的驗(yàn)證。

    Abstract:

    Developing portable devices for extraction and detection of oxytetracycline (OTC) in soil, and developing accurate methods for on-site analysis of OTC in soil is of great importance to ensure the safety of agricultural environment and human health. The existing devices for OTC extraction require additional devices to meet the needs for on-site extraction, and the low sensitivity of the on-site detection method limits its application in accurate detection of trace OTC in soil. Integration circuits provide ideas of hardware integration design for soil OTC extraction-detection devices because of the advantages of simple circuitry, low failure rate, low power consumption, and high reliability;the photoelectrochemical (PEC) aptasensor is expected to achieve accurate detection of OTC for its high sensitivity, good selectivity, and easy portability. A portable device was developed for extraction and PEC detection of OTC in soil by designing an integrated hardware circuit. Firstly, a portable device for extraction of OTC in soil was designed and developed with STM32F103ZET6 as the control core. The extraction of OTC from soil was accomplished using the developed portable device and laboratory device, respectively, and the extraction precision was verified by using ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS/MS). Subsequently, a portable PEC detection device was prepared by using integrated Bi2S3-C3N4 based screen-printed electrodes, low-power LEDs, and a miniaturized electrochemical workstation. The design of hardware circuit and light source darkroom had the function of controlling the timing switching of the LEDs during PEC detection and avoiding the interference of the external light source on the output PEC signal. Lastly, the portable extraction-PEC detection device was used for on-site analysis of OTC in soil. Results showed that the extraction precision of the developed portable device was accordance with that of the laboratory device;and the portable PEC detection device had a linear range of 1×10-8~1×10-6mol/L and a detection limit of 5.33×10-9mol/L was obtained for OTC detection;and the results of the on-site analytical method for OTC were basically agree with the results of the UPLC-MS/MS method. This work would realize the on-site analysis of OTC in soil and provide technological support for soil treatment.

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張航,高信,劉倩,由天艷.便攜式土壤土霉素提取-檢測裝置設(shè)計(jì)與試驗(yàn)[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2024,55(4):32-39. ZHANG Hang, GAO Xin, LIU Qian, YOU Tianyan. Design and Experiment on Portable Device for Extraction and Detection of Oxytetrarycline in Soil[J]. Transactions of the Chinese Society for Agricultural Machinery,2024,55(4):32-39.

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  • 收稿日期:2023-12-06
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  • 在線發(fā)布日期: 2024-04-10
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