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The shape of Cyperus esculentus seeds is irregular and the surface is uneven. Most of the domestic seed equipment of the seed ball is improved based on the large seed ball seed feeder. Due to the lack of accurate simulation parameters during the discrete element simulation experiment, this results in errors in the simulation and optimization analysis of the seed dispenser. To solve this problem, the intrinsic parameters and the contact parameters between seeds and seeding device were measured, and the seed particle filling model was constructed by reverse engineering technology. According to the results of simulation accumulation test of square shell and cylinder, Plackett-Burman test was used to screen out the interspecific static friction coefficient and interspecific rolling friction coefficient which had significant influence on population accumulation angle. A binary regression equation was established with these two significant influence factors as independent variables and population accumulation angle as response value. After solving the equations with Matlab, the calibrated interspecific static friction coefficient and interspecific rolling friction coefficient were 0.246 and 0.083, respectively. To verify the reliability of parameters after calibration, simulation verification test was carried out in EDEM, and the results showed that the error between simulation and physical accumulation angle of square shell device and physical accumulation angle of cylinder device was 0.43%, and the error between simulation and physical accumulation angle of cylinder device was 0.55%. To further verify the accuracy of parameters after calibration, the pneumatic precision seed disperser was used and the three key feature sizes and the ratio of stack crosssectional area were selected. After Cyperus esculentus seeds was stabilized, the simulation and physical test were compared, finally, the relative errors of the parameters H1 from the maximum convex point of the upper boundary of the population to the horizontal center line of the seed platen, H2 from the intersection point of the population profile boundary and the vertical line on the left side of the transparent observation window to the horizontal center line of the seed platen, and the ratio r of the accumulation crosssectional area were all within 8.33%.