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At present, there were few studies on the influence of the soybean height on the parameter of reel of soybean combine harvester in China, while the height of soybean plants in different varieties and producing areas varies greatly. In order to change the current situation of soybean combine harvester, due to the lack of theoretical guidance for the parameter adjustment of the reel, the reel parameter is not timely and accurate, resulting in high loss rate of soybean combine harvester harder. ANSYS and ADAMS co-simulation method was used to optimize the parameters of the reel of soybean combine harvester when harvesting soybeans at different heights. The influence rules of the three reel parameters and soybean plant height on the two header loss indexes were analyzed, and the best reel parameter combination at different height was found. The field verification test of the best reel parameter combination of soybean harvester was completed. Based on the analysis of the structure of reel and the principle of reel operation of soybean harvester, the importance of the reel speed ratio, reel height and reel forward displacement to the header loss indexes of soybean combine harvester was obtained. The reel speed ratio, reel height, reel forward displacement and the soybean plant height were used to optimize the four parameters in simulation test. Collision force of reel to soybean stem and effect degree of reel were used to optimize the two indexes of simulation test. The model of test indexes under test factors was established. The objective optimization equations were established. There was linear relationship between optimal parameters of reel of soybean combine harvester and soybean plant height. The order of influence of soybean combine harvester reel parameters on the two indexes was as follows: reel speed ratio, reel height, and reel forward displacement. The simulation test was carried out with the collision force of the reel to the soybean stem and the degree of action of the reel as the index, and the field test with the loss rate of the header as the index in the case of soybean plant height of 751.5mm. The average deviation of the collision force between the model calculation and the simulation was 1.18N and the average deviation of the effect degree of reel was 4.80%, which showed that the simulation model was accurate. The optimal parameters combination of reel was reel speed ratio of 1.66, reel forward displacement of 40mm and reel height of 1052mm. This solution as field experiment factor had the smallest loss rate in the experiment. The research results can provide a basis for the optimization of the allocation reel parameters of soybean combined harvest at different heights and the reduction of cutting loss.