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For the plug-in seedling picking mechanism, when the seedling claws are inserted into the pot to pick up the seedlings, the success rate of seedling picking is low and the pot is broken due to the double influence of the adhesion between the pot and the plug and the poor packing. In order to solve the problem of high rate, a combined seedling picking technology with top clamping and pulling was proposed, the structure and working principle of the seedling picking device were expounded, and the experimental research on the combined top clamping and pulling seedling picking was carried out. Firstly, the 72-hole and 128-hole cucumber plug seedlings were used as the test objects, and the adhesion of the cucumber seedlings at different top seedling speeds (10mm/s, 20mm/s, 30mm/s and 40mm/s) was tested through the top pressure off-disk adhesion test. The test results showed that the top seedling speed had little effect on the adhesion force and the detachment displacement of the seedling pot, and the adhesion force was positively correlated with the detachment displacement of the seedling pot. The value was distributed between 5.5mm and 6.9mm. Considering the diameter of the seedling tray drain hole and the test results of the top pressure off tray adhesion test, it was determined that the diameter of the mandrel was 6mm, and the displacement of the top seedling of the mandrel must be greater than 5mm. Secondly, taking the 72-hole cucumber plug seedlings with a growth cycle of 25d as the test object, three modes of seedling collection were carried out, namely, the first top and then the top, the side top and the side, and the first plug and then the top. The seedling success rate and pot body integrity rate were the highest. Finally, taking the ejection displacement of the ejector rod, the depth of inserting the seedling claws into the seedling pot and the speed of inserting the seedlings as the test factors, a three-factor and three-level orthogonal test was carried out. The optimal working parameter combination was that the jacking displacement was 15mm, the depth of inserting the seedling pot was 35mm, and the speed of inserting the seedling was 225mm/s. The success rate of seedling removal under this combination was 94.12%, and the complete rate of the seedling pot was 94.12%, which satisfied the automatic seedling quality requirements.