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冰溫貯藏甘南藏羊肉色穩(wěn)定性與線粒體MMb還原能力研究
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甘肅省重點(diǎn)研發(fā)計(jì)劃項(xiàng)目(20YF3NA014)和甘肅省高等學(xué)校創(chuàng)新基金項(xiàng)目(2020A-049)


Color Stability and Mitochondrial MMb Reducing Ability of Gannan Tibetan Sheep Meat by Freezing Point Storage
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    摘要:

    為確定冰溫貯藏甘南藏羊肉色穩(wěn)定性與線粒體MMb還原能力及其相關(guān)性,,以藏羊后腿肉為試驗(yàn)材料,,分別在冰溫((-1.4±0.1)℃)和冷藏((4±1)℃)條件下貯藏,測定貯藏期間(0,、1,、3、5,、7,、9d)MRA、NADH含量,、SDH活力,、線粒體膜通透性、線粒體膜電位與色調(diào)角的變化,。結(jié)果表明,,貯藏9d時(shí),冰溫組MRA,、NADH含量,、線粒體膜540nm處吸光度、線粒體膜電位,、SDH活力比對照組分別高16.67%,、33.16%、25.45%,、16.57%,、40.45%(P<0.05),冰溫組色調(diào)角比冷藏組低13.13%(P<0.05),。冰溫組,、冷藏組的MRA、NADH含量,、線粒體膜540nm處吸光度,、線粒體膜電位、SDH活力均與色調(diào)角呈極顯著負(fù)相關(guān)(P<0.01),。由此可見,,冰溫貯藏可延緩MRA、NADH含量的下降,,同時(shí)維持線粒體結(jié)構(gòu)的完整性,,最終有利于宰后肉色的穩(wěn)定,為改善藏羊肉色穩(wěn)定提供了理論依據(jù),。

    Abstract:

    In order to clarify the effect of controlled freezing point storage on color stability and mitochondria metmyoglobin reduction ability of Tibetan sheep meat, the hind legs meat of Tibetan sheep were used as the material, and Tibetan sheep meat was controlled by freezing point storage and cold storage, and the duration of storage for 0d, 1d, 3d, 5d, 7d and 9d. The changes in MRA, NADH content, SDH activity, mitochondrial membrane permeability, mitochondrial membrane potential, Hue angle duration of storage were determined. The results showed that after aging of 9d, controlled freezing point storage group MRA, NADH content, mitochondrial membrane permeability, mitochondrial membrane potential, SDH activity were significantly 16.67%,33.16%,25.45%,16.57% and 40.45% higher than that of the cold storage group (P<0.05), Hue angle of controlled freezing point storage group were significantly 13.13% lower than that of the cold storage group (P<0.05). MRA, NADH content, SDH activity, mitochondrial absorbance at 540nm, mitochondrial membrane permeability of controlled freezing point storage and cold group were negatively correlated with Hue angle values (P<0.01). In conclusion, controlled freezing point storage could delay the decrease of MRA and NADH content, and maintain the integrity of mitochondrial structure, which were ultimately conducive to the stability of postmortdial meat color. The research results provided a theoretical basis for improving the color stability of Tibetan sheep meat.

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師希雄,張攀高,趙瑞娜,包曉明,范小寧.冰溫貯藏甘南藏羊肉色穩(wěn)定性與線粒體MMb還原能力研究[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2022,53(5):400-405. SHI Xixiong, ZHANG Pan’gao, ZHAO Ruina, BAO Xiaoming, FAN Xiaoning. Color Stability and Mitochondrial MMb Reducing Ability of Gannan Tibetan Sheep Meat by Freezing Point Storage[J]. Transactions of the Chinese Society for Agricultural Machinery,2022,53(5):400-405.

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  • 收稿日期:2022-01-04
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  • 在線發(fā)布日期: 2022-05-10
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