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酶法催化紫蘇油和MCT合成中長(zhǎng)鏈甘油三酯研究
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國(guó)家重點(diǎn)研發(fā)計(jì)劃項(xiàng)目(2016YFD0401405)、山東省重點(diǎn)研發(fā)計(jì)劃項(xiàng)目(2018YYSP026),、山東省德州市大禹英才項(xiàng)目和山東省泰山產(chǎn)業(yè)領(lǐng)軍人才工程高校生態(tài)農(nóng)業(yè)創(chuàng)新類項(xiàng)目(LJNY201607)


Enzymatic Synthesis of Medium- and Long-chain Triacylglycerols Based on Perilla Oil and MCTs
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    摘要:

    通過(guò)Lipozyme RM IM催化紫蘇油與中鏈甘油三酯(MCT)進(jìn)行酯交換合成富含α-亞麻酸的中長(zhǎng)鏈甘油三酯(MLCT),,研究了酶添加量、底物中MCT濃度,、反應(yīng)溫度和反應(yīng)時(shí)間對(duì)MLCT得率的影響,。40%MCT和60%紫蘇油在6%的Lipozyme RM IM催化作用下,于60℃進(jìn)行4h的酯交換反應(yīng),,結(jié)果表明,,在反應(yīng)平衡后,MLCT得率超過(guò)70%,。采用兩步法除臭工藝純化酯交換產(chǎn)物,,產(chǎn)品中的甘油三酯純度達(dá)到97%,游離脂肪酸含量顯著降低,,反式脂肪酸質(zhì)量分?jǐn)?shù)可以控制在1%以內(nèi)。此外,,在純化產(chǎn)物中存在超過(guò)40%的α-亞麻酸,,超過(guò)85%的長(zhǎng)鏈脂肪酸在甘油三酯中的sn-2位置。

    Abstract:

    The medium- and long-chain triacylglycerols (MLCTs) rich in α-linolenic acid could be used as an anti-obesity, functional material in food industry. MLCTs could overcome the shortcomings of the LCTs and MCTs, and provide the human body with essential fatty acids and faster energy supply. Enzymatic interesterification of perilla oil and MCTs was performed to prepare the MLCTs rich in α-linolenic acid, the reaction were catalysed by Lipozyme RM IM. The effects of enzyme loading, ratio of MCTs to perilla oil, reaction time and reaction temperature on the yield of MLCT were studied. The results showed that the reaction achieved more than 70% yield of MLCTs in triacylglycerols under the conditions of 6% Lipozyme RM IM, 40% MCT and 60% perilla oil at 60℃ for 4h. Deodorization was a traditional deacidification method, in consideration of processing costs and equipment reconstruction costs, the two-stages deodorization was used to update into a two-stages deodorization to better remove the undesired by-products after interesterification reaction, providing a method for the physical deacidification of polyunsaturated oils in practice. The free fatty acids of the interesterified products were removed significantly, the triacylglycerols were more than 97% in the refined products, and the trans fatty acids could be limited within 1%. In the purified products, more than 85% of the long-chain fatty acids were occupying the sn-2 position in triacylglycerols, and the total α-linolenic were more than 40%.

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黃昭先,王滿意,孫承國(guó),王風(fēng)艷,王翔宇,江連洲.酶法催化紫蘇油和MCT合成中長(zhǎng)鏈甘油三酯研究[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2021,52(1):360-366,359. HUANG Zhaoxian, WANG Manyi, SUN Chengguo, WANG Fengyan, WANG Xiangyu, JIANG Lianzhou. Enzymatic Synthesis of Medium- and Long-chain Triacylglycerols Based on Perilla Oil and MCTs[J]. Transactions of the Chinese Society for Agricultural Machinery,2021,52(1):360-366,,359.

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  • 收稿日期:2020-10-06
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  • 在線發(fā)布日期: 2021-01-10
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