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基于高光譜成像的生鮮雞肉糜中大豆蛋白含量檢測
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國家自然科學(xué)基金面上項目(31772062)和國家重點研發(fā)計劃項目(2018YFC1603500)


Detection of Soybean Protein Content in Fresh Minced Chicken Meat Using Hyperspectral Imaging
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    摘要:

    為快速無損檢測生鮮雞肉糜中超量添加的大豆蛋白含量,采集了雞肉糜中摻雜的3種典型大豆蛋白的高光譜圖像,。在雞肉糜中分別摻入質(zhì)量分?jǐn)?shù)0~30%的大豆蛋白粉(SPF),、大豆?jié)饪s蛋白(SPC)和大豆分離蛋白(SPI),,并于可見-近紅外(400~1000nm)光譜范圍采集樣品的高光譜圖像,,基于波段運(yùn)算提取感興趣區(qū)域的平均光譜,建立了原始及預(yù)處理光譜的偏最小二乘回歸(PLSR)定量模型,,發(fā)現(xiàn)模型對SPC預(yù)測效果最優(yōu)(R2p=0.9984),、SPF次之、SPI最差,。進(jìn)一步利用二維相關(guān)光譜(2DCOS)自相關(guān)峰提取特征波長,,建立的多光譜模型對于3種大豆蛋白的檢測限分別可達(dá)0.53%、0.58%和1.02%,。將以上多光譜模型應(yīng)用到原始光譜圖像,,實現(xiàn)了不同大豆蛋白及其摻假梯度的可視化表征。

    Abstract:

    In order to rapidly and nondestructively detect the excessive soybean protein content in fresh minced chicken meat, hyperspectral images of three typical soybean protein adulterated in minced chicken meat were collected. Soybean protein flour (SPF), soybean protein concentrate (SPC) and soybean protein isolates (SPI) with 0~30% adulteration levels were mixed into the minced chicken meat. The hyperspectral images of prepared samples were collected in the visible and near infrared (400~1000nm) spectral range. The average spectra of regions of interest were extracted based on band operation and models based on the original and preprocessed spectra were established. Partial least squares regression (PLSR) quantitative models showed that the optimal performance were best for SPC (R2p=0.9984), followed by SPF, and then SPI. The twodimensional correlation spectrum (2DCOS) was used to extract the characteristic wavelengths to further establish multispectral models. The limits of detection (LODs) of the multispectral models were found to be 0.53%, 0.58% and 1.02% for SPF, SPC and SPI, respectively. The multispectral models above were also applied back to the raw multispectral images to visualize the distribution of different soybean proteins, as well as their adulteration levels. Results showed that the distribution differences within samples and among samples could be well visualized.

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王偉,姜洪喆,賈貝貝,鹿瑤.基于高光譜成像的生鮮雞肉糜中大豆蛋白含量檢測[J].農(nóng)業(yè)機(jī)械學(xué)報,2019,50(12):357-364. WANG Wei, JIANG Hongzhe, JIA Beibei, LU Yao. Detection of Soybean Protein Content in Fresh Minced Chicken Meat Using Hyperspectral Imaging[J]. Transactions of the Chinese Society for Agricultural Machinery,2019,50(12):357-364.

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  • 收稿日期:2019-07-24
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  • 在線發(fā)布日期: 2019-12-10
  • 出版日期: 2019-12-10
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