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便攜式植物葉片葉綠素含量無(wú)損檢測(cè)儀設(shè)計(jì)與試驗(yàn)
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公益性行業(yè)(農(nóng)業(yè))科研專項(xiàng)(201503137)和國(guó)家級(jí)大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計(jì)劃項(xiàng)目(201710712051)


Design of Portable Nondestructive Detector for Chlorophyll Content of Plant Leaves
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    摘要:

    為了快速、無(wú)損檢測(cè)植物葉片葉綠素含量,基于葉綠素a和葉綠素b在光波長(zhǎng)約660nm和460nm處有最大吸收峰的現(xiàn)象,設(shè)計(jì)了一種便攜式植物葉片葉綠素含量無(wú)損檢測(cè)儀,。該檢測(cè)儀主要由單片機(jī),、光源模塊,、光傳感器,、電源模塊和輸入輸出模塊等組成,;其軟件采用Keil C51編寫(xiě),,主要包括主函數(shù),、按鍵子函數(shù)、光采集子函數(shù),、數(shù)據(jù)處理子函數(shù),、顯示子函數(shù)等。以菠菜,、大青菜和油麥菜為試驗(yàn)對(duì)象,,研究了460nm和660nm處植物葉片的吸光度與葉綠素含量之間的關(guān)系,結(jié)果表明,,隨著葉綠素含量的增加,,吸光度增大,其關(guān)系可用二元一次方程描述(決定系數(shù)為080),。與分光光度法相比,,本文設(shè)計(jì)檢測(cè)儀的葉綠素含量檢測(cè)誤差為-0.32~0.20mg/g,平均絕對(duì)誤差為0.14mg/g,;與SPAD-502型葉綠素儀相比,,本文設(shè)計(jì)檢測(cè)儀的SPAD值絕對(duì)測(cè)量誤差為-3.3~1.8,平均絕對(duì)誤差為1.1,,且成本低,,響應(yīng)時(shí)間小于2s。

    Abstract:

    As the most essential pigment included in green plants, chlorophyll content is an important indicator in monitoring the growth of plants. The traditional spectrophotometric method used to measure the chlorophyll content of plant leaves has the advantages of high measurement accuracy and good repeatability, but the measurement process are timeconsuming. Therefore, developing a portable rapid chlorophyll detector with simple operation and low cost, and could be used insitu measurement will be appreciated by agricultural and forestry practitioners. Since the absorption peaks of chlorophyll a and b appeared at 660nm and 460nm, a portable chlorophyll content detector was developed based on this phenomenon. The hardware of the detector consisted of a single chip microcomputer, light sources module, light sensors, power module, and input and output module. The software, developed by using Keil C51 language, including main function and several subfunctions to detect the light, process data, calculate the chlorophyll content and display the values on screen. The leaves of spinach, big green vegetables and lettuce were used as samples, and the relationship between their chlorophyll contents and light absorbance at 460nm and 660nm were studied. It was found that the absorbance was increased with the increase of chlorophyll content, and their relationship could be described by first degree in two variable equation with the coefficient of determination of 080. The validation experiment on the developed detector showed that contrasted with the spectrophotometer method, the absolute measurement error of the developed detector was within the range of -0.32~0.20mg/g, and its mean absolute error was 0.14mg/g. Contrasted with the measured SPAD values by using SPAD-502 chlorophyll meter, the measurement error of the SPAD values measured by the developed detector was within the range of -3.3~1.8, and its mean absolute error was 1.1. Moreover, the measurement time of the detector was less than 2s and the cost of the detector was not high.

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楊彪,杜榮宇,楊玉,朱德寬,郭文川,朱新華.便攜式植物葉片葉綠素含量無(wú)損檢測(cè)儀設(shè)計(jì)與試驗(yàn)[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2019,50(12):180-186. YANG Biao, DU Rongyu, YANG Yu, ZHU Dekuan, GUO Wenchuan, ZHU Xinhua. Design of Portable Nondestructive Detector for Chlorophyll Content of Plant Leaves[J]. Transactions of the Chinese Society for Agricultural Machinery,2019,50(12):180-186.

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  • 收稿日期:2019-05-06
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  • 在線發(fā)布日期: 2019-12-10
  • 出版日期: 2019-12-10
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