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Aiming at the problems of manpower consumption and low picking efficiency of Hangzhou white chrysanthemum, a small and practical combtooth reciprocating device for Hangzhou white chrysanthemum picking was designed. According to the growth characteristics and picking abstracts of Hangzhou white chrysanthemum, the structure of the picking device was modeled by SolidWorks software, and the picking device was built. The device utilized comb stripping and rolling brushing cleaning functions of comb and the quick return characteristics of crankrocker mechanism to achieve the harvesting of Chrysanthemum morifolium. The physical and mechanical properties of Chrysanthemum morifolium were tested. In order to obtain the optimum working parameters of the device, field tests were carried out. Firstly, through single factor picking test, three main factors affecting picking effect were determined, namely, comb reciprocating frequency, comb spacing and comb working depth. Then, with comb reciprocating frequency, comb spacing and comb working depth as experimental factors, the orthogonal test was carried out to evaluate the picking rate, impurity content, breakage rate and landing rate of Chrysanthemum morifolium. Under the combination of reciprocating frequency of comb as 40r/min, distance of comb as 6.5mm and working depth of comb as 200mm, the picking effect was the best. At this time, the picking rate of Chrysanthemum morifolium was 92.47%, the impurity rate was 11.07%, the damage rate was 1.48%, and the landing rate was 1.41%. The comb reciprocating picking device of Hangzhou white chrysanthemum achieved good picking effect.