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Aquatic products have high economic benefits and are prone to spoilage, which is mainly related to enzyme activity in the body, and temperature is an important reason for affecting enzyme activity. Among them, live aquatic products require higher temperature stability. Thus how to maintain uniform temperature in refrigerated compartments and ensure the quality of aquatic products has become an important research topic, in which the way of goods stacking is an important research direction. The distribution of temperature field inside the carriage of the refrigerated aquatic products with upper air outlet and upper air return was simulated by using Fluent module in ANSYS 16.0. The temperature cloud diagrams of the surface, cross section and longitudinal section of the cargo under the refrigeration device were compared with those under the four stacking modes, which were 0m, 0.533m (H/3), 1.066m (2H/3) and 1.6m (H). The results showed that the temperature distribution was in the form of inverted “ C ” along the length direction of the carriage, that was, the area near the lower part and the rear lower part of the carriage was higher;the goods stacked under the refrigeration device would have a significant impact on the uniformity of the temperature distribution. In the case of stacking mode a when the stack height was 0m, the temperature distribution was more uniform, the temperature in the cargo area was within 5℃, and the maximum temperature difference was 2℃. Comparing the experimental results with the simulation results, the maximum root mean square error of the two results was less than 0.5℃, and the maximum average relative error was 7%. Test results and simulation results showed good continuity. The general law of temperature field distribution in refrigerated aquatic products transport carriage was obtained, which had a certain degree of practical guiding significance for the cargo layout and structure optimization of aquatic products transport vehicle.