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The leaching loss of soil nutrients along with water is serious in the process of agricultural planting, it not only wastes fertilizer resources, but also causes eutrophication of surface water and further threatens groundwater quality, and the phenomenon is more serious during the application of liquid bioorganic fertilizer biogas slurry. Biochar has strong adsorption due to its special physical and chemical properties, adding biochar to soil to reduce the loss of nutrient has become a research focus. However, its leaching process and law still need to be further studied. To investigate the effects of soil nutrient leaching after biogas slurry was applied under different conditions, the orthogonal design of threefactor and threelevel L9 (33) was adopted through indoor soil column simulation test. The effects of biochar addition amount（2%, 5% and 10%）, leaching intensity（46mL, 137mL and 250mL） and biogas slurry addition amount（100mL, 200mL and 300mL）on soil nutrient leaching and vertical distribution of soil nutrients after leaching were systematically studied. The results showed that soil nutrient leaching was mainly concentrated in the early stage of leaching (leaching was mainly concentrated in the first five times of leaching), the leaching loss amount remained at a low level and gradually became stable at later stage. The effects of various factors on the leaching loss of ammonia nitrogen, rapidly available phosphorus and rapidly available potassium were successively the leaching strength, amount of biochar added and amount of biogas slurry applied, however, the effects on the loss amount of nitrate nitrogen leaching were successively the amount of biochar, amount of biogas slurry and leaching intensity. Compared with the control group, the nutrient leaching loss of biochar treatment was significantly reduced. The content of various nutrients of the soil with depth of 0~20cm added with biochar was significantly higher than that of the soil with depth of 20~40cm without biochar. The effects of various factors on the content of ammonia nitrogen, nitrate nitrogen and rapidly available potassium in soil were significantly different in longitudinal depth, but there was no significant difference on the content of rapidly available phosphorus. The experiment laid a theoretical foundation for the research on leaching effect of biochar addition and biogas slurry application on soil nutrients, the research and application of biochar as a soil improvement additive as well as the scientific and reasonable application of biogas slurry in the field production can be accelerated.