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CaCl2對(duì)纖維核誘導(dǎo)乳清濃縮蛋白聚合能力的影響
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國(guó)家自然科學(xué)基金項(xiàng)目(31471682)


Effect of CaCl2 on Aggregates of Whey Protein Concentrate with Adding Fibril Nuclei
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    摘要:

    研究了CaCl2對(duì)兩種核誘導(dǎo)乳清濃縮蛋白(WPC)在聚合方面的影響,。通過向WPC自發(fā)、均相核誘導(dǎo)WPC,、二次核誘導(dǎo)WPC形成纖維過程中添加CaCl2,,可提高纖維體系的硬度、黏度以及聚合率,,并且對(duì)核誘導(dǎo)形成的纖維聚合物影響較大,。添加CaCl2后,核誘導(dǎo)的硬度增加量是WPC自發(fā)硬度增加量的1.661倍(均相核誘導(dǎo))和1.821倍(二次核誘導(dǎo)),,黏度增加量的對(duì)應(yīng)值為2.261倍(均相核誘導(dǎo))與2.479倍(二次核誘導(dǎo)),。添加CaCl2使纖維的形態(tài)發(fā)生變化,,成彎曲聚集體。對(duì)于纖維形成的兩種路徑自發(fā)與核誘導(dǎo),,CaCl2的影響程度不同,,與乳清濃縮蛋白自發(fā)纖維聚合能力相比,CaCl2對(duì)核誘導(dǎo)乳清濃縮蛋白聚合能力的提升幅度更大,。

    Abstract:

    Whey protein concentrate (WPC) fibril was made at pH value of 2.0 and 90℃. The investigation focused on the impact of adding CaCl2 on the aggregation of WPC spontaneous, homogeneous nuclei induced WPC and secondary nuclei induced WPC. As adding CaCl2, the properties of the hardness, viscosity and amount of WPC fibril aggregates with formation spontaneous or induced homogeneous/secondary nuclei were all increased, especially for two nuclei induced whey concentrate proteins. Adding CaCl2, the hardness was increased by 1.661 (homogeneous nuclei induced WPC) and 1.821 (secondary nuclei induced WPC) times than that of whey protein concentrate spontaneous, and the viscosity was increased by 2.261 (homogeneous nuclei induced WPC) and 2.479 (secondary nuclei induced WPC) times. The micro morphology using TEM of WPC aggregates was changed from long and semi-flexible fibrils into curly and cluster polymers when mixed CaCl2. And the fluorescence intensity was all decreased by adding CaCl2. It was speculated that because the Th T dye was unable to fully bind with the assembled fibrils. For the two ways of forming fibrils: spontaneous or nuclei induced WPC, there were different results that the aggregation ability of nuclei-induced WPC was even greater than that of spontaneous WPC fibril formation as adding CaCl2.

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徐紅華,謝明明,丁瑞,馬金玉,高子雯,關(guān)琛. CaCl2對(duì)纖維核誘導(dǎo)乳清濃縮蛋白聚合能力的影響[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2018,49(10):375-380. XU Honghua, XIE Mingming, DING Rui, MA Jinyu, GAO Ziwen, GUAN Chen. Effect of CaCl2 on Aggregates of Whey Protein Concentrate with Adding Fibril Nuclei[J]. Transactions of the Chinese Society for Agricultural Machinery,2018,49(10):375-380.

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  • 收稿日期:2018-07-05
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  • 在線發(fā)布日期: 2018-10-10
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