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紅肉火龍果采后病原菌分離鑒定與植物精油抑菌研究
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國(guó)家重點(diǎn)研發(fā)計(jì)劃項(xiàng)目(2017YFD0401301,、2018YFD0401304)和國(guó)家自然科學(xué)基金項(xiàng)目(31701658)


Isolation, Identification and Inhibition of Pathogens from Red Pitaya Fruit during Storage
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    摘要:

    分離、純化并鑒定了浙江產(chǎn)區(qū)紅肉火龍果貯藏期病原菌,,篩選出對(duì)抑制病原菌效果較好的精油,,并驗(yàn)證了該精油抑菌效果。通過分離、純化及致病性檢測(cè),得到了病原菌菌株HS3和HS6,。以病原菌18S rDNA序列進(jìn)行進(jìn)化樹分析,確定兩種病原菌為層生鐮刀菌和平頭炭疽菌,。選擇6種植物精油進(jìn)行抑菌性試驗(yàn),結(jié)果發(fā)現(xiàn)香芹酚精油,、肉桂精油及百里香精油在2000μL/L時(shí)對(duì)兩種病原菌生長(zhǎng)抑制率均為100%,。采用體外直接接觸法和體外熏蒸法,研究了香芹酚精油,、肉桂精油和百里香精油對(duì)兩種病原菌的抑制效果,,發(fā)現(xiàn)香芹酚精油表現(xiàn)出較強(qiáng)的抑菌性。無論直接接觸還是熏蒸方式,,香芹酚精油對(duì)層生鐮刀菌和平頭炭疽菌的抑菌效果均優(yōu)于肉桂精油和百里香精油,。精油熏蒸處理使接種層生鐮刀菌和平頭炭疽菌火龍果腐爛率下降,香芹酚精油的效果最優(yōu),,可作為火龍果貯藏前的熏蒸劑,。研究結(jié)果可為浙江地區(qū)火龍果貯藏期腐爛病害的預(yù)防和控制提供一定的參考依據(jù)。

    Abstract:

    Red pitaya fruit is highly susceptible to fungal infection during postharvest, causing quality deterioration and huge economic losses. This research was intended to isolate, purify and identify the pathogens which cause rotting of red pitaya during storage in Zhejiang Province, screen essential oils for controlling the pathogenic isolates, and test their effectiveness. HS3 and HS6 were identified as pathogens through isolation, purification and pathogenicity tests. These two strains were proved as F. proliferatumandC. fructicola by morphological identification and rDNA-ITS molecular identification. Six essential oils were investigated for their activity to control these pathogens. The result indicated that carvacrol, cinnamon and thyme oil showed complete inhibitory effect on all pathogens at a concentration of 2000μL/L. Then, the inhibitory effects of carcanol, cinnamon and thyme oil on these two pathogens were studied by direct contact in vitro and in vitro fumigation. It was found that carcanol showed higher antifungal activity against F. proliferatum and C. fructicola than cinnamon and thyme in the drug sensitivity test. Moreover, essential oil fumigation inhibited the decay incidence of pitaya fruit inoculated with F. proliferatum and C. fructicola during storage at 4℃ and carcanol showed the best effect. Carcanol oil seemed to be a promising potential fumigant. The results can provide reference for disease control during storage of red pitaya in Zhejiang Province.

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劉瑞玲,郜海燕,陳杭君,房祥軍,吳偉杰.紅肉火龍果采后病原菌分離鑒定與植物精油抑菌研究[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2018,49(8):338-345. LIU Ruiling, GAO Haiyan, CHEN Hangjun, FANG Xiangjun, WU Weijie. Isolation, Identification and Inhibition of Pathogens from Red Pitaya Fruit during Storage[J]. Transactions of the Chinese Society for Agricultural Machinery,2018,49(8):338-345.

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  • 收稿日期:2018-05-01
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  • 在線發(fā)布日期: 2018-08-10
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