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Since early 1990s, the agricultural structure of Naoli River Basin had been greatly adjusted with large amounts of dry land changed into paddy field. As one of the important reclamation farms in China, the land use was changed severely in Naoli River Basin and such phenomenon would lead to a set of problems in the utilization. To reveal the spatial regular pattern and the difference of land use change in Naoli River Basin, the land use dynamic changes were analyzed, and the land use dynamic degree of the cultivated land was calculated by modeling all land use types of cultivated land and using the contribution results which was borrowed from the thought of landscape ecology, the analysis of land use spatial autocorrelation patterns was also achieved based on sub basin of Naoli River Basin. The remote sensing image data and DEM data were used as fundamental data resources. Results showed that the land use changes in the Naoli River Basin were incredibly violent, the increase of farmland area was the largest, and the internal transformations between the paddy field and dry land were the primary change. From 1990 to 2002, the change of paddy field was the main change types and the dynamic degree of land use change was obviously stronger than that during the period from 2002 to 2014. The middle and northern parts of Naoli River Basin were the mainly concentrated region of high land use dynamic degree, and the overall changing speed of the river basin was slowly trending down. The dynamic degree of land use change in different directions had good fitting trend and differed obviously between the two periods, but it maintained the basic trend that the change in the northeast direction was significantly higher than that in the southwest direction. The whole catchment previously showed the trend of aggregation of high to high and low to low obviously, the land use spatial autocorrelation patterns showed significant correlation, but in the later stage, the aggregation trend was gradually slowed down, and its aggregate on features was high to high and low to low, there was no catchment with aggregation trend of high to low. The research results can be used as references and consultancies for the land use zoning management and precise agriculture in the Naoli River Basin.