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The scanning Raman imaging detection system was constructed, and the Raman spectrum and Raman image can be acquired. In accordance with the actual needs and the detection accuracy, the Raman imaging spectrometer, CCD cameras, lenses, laser light, mobile translation stage and other major components were selected. The real-time detection and analysis software of the Raman imaging detection system was developed by using LabView programming. The detection software interface was designed by applying modular and integrated design ideas to the LabView-based software development environment. By using the software, the CCD camera, laser light source and mobile translation stage controls, parameter settings and hardware detection state monitoring can be achieved, real-time collection and display of synthetic Raman image of test sample and Raman spectral curve dynamic display can also be completed. Data acquisition, analysis and calculation results saving were completed by using Matlab algorithms in LabView. The image synthesis and real-time display were completed using ENVI programming in LabView. One-button operation design was used for the software and it had the advantages of simple interface, easy operation and software portability. By combining with the software, the hardware detection system can totally meet the requirement of real-time analysis and application. Then, the system was installed and its performance was tested. The corrected spectral CCD camera can detect the Raman spectral range of -679.3~2885.7cm-1. The system ensured the actual spatial resolution was 0.22mm/pixel via space calibration, stability of test performed well, and Raman images acquisition can be achieved.