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寧夏枸杞園土壤線蟲和微生物群落多樣性研究
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國家自然科學(xué)基金項(xiàng)目(41261080)和寧夏自治區(qū)環(huán)保專項(xiàng)


Soil Nematode and Microbial Community Diversity in Lycium barbarum L. Orchard
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    摘要:

    為了揭示寧夏枸杞園土壤質(zhì)量變化趨勢,,以寧夏枸杞之鄉(xiāng)——中寧縣為研究區(qū),,選取不同樹齡寧夏枸杞園土壤,分析不同季節(jié),、土層和樹齡條件下土壤線蟲和微生物群落特征變化規(guī)律,。結(jié)果表明,,隨樹齡的增加,,枸杞園0~20cm土壤線蟲總數(shù)先增加后減少,6a樹齡時達(dá)最大值,。不同樹齡表層土壤均為食細(xì)菌線蟲所占比例最大(夏季和秋季平均分別為57.23%和61.19%),,植物寄生線蟲次之;亞表層植物寄生線蟲比例顯著提高,。夏季表層土壤總磷脂脂肪酸(PLFA)和細(xì)菌PLFA濃度隨樹齡呈減小-增大-減小的趨勢,,9a樹齡各菌群PLFA濃度普遍最大。隨樹齡的增加,,夏季0~20cm土壤線蟲多樣性和豐富度指數(shù)先增大后減小,,但土壤微生物多樣性指數(shù)、均勻度指數(shù)都逐漸減小,,線蟲和微生物群落優(yōu)勢度指數(shù)都逐漸增大,;20~40cm土壤線蟲和微生物數(shù)量、多樣性指數(shù)和優(yōu)勢度指數(shù)變化趨勢一致,。土壤EC與微生物總PLFA濃度,、細(xì)菌PLFA濃度達(dá)顯著負(fù)相關(guān);土壤有機(jī)質(zhì),、全氮,、速效磷含量與土壤各線蟲數(shù)量和微生物濃度相關(guān)性普遍達(dá)顯著或極顯著水平。土壤線蟲總數(shù),、食細(xì)菌線蟲數(shù)量與細(xì)菌,、真菌和放線菌PLFA濃度均顯著或極顯著相關(guān)??傊?,季節(jié)、土層和樹齡對土壤線蟲和微生物群落均有不同程度的影響,,但樹齡對其影響相對最?。患竟?jié),、土層和樹齡對土壤微生物群落的影響比對土壤線蟲群落更顯著,。在相同季節(jié)和土層條件下,土壤微環(huán)境質(zhì)量隨著樹齡的增加呈現(xiàn)出先改善后退化的趨勢,。

    Abstract:

    Soil nematode and microbes are essential and very sensitive to any upsets in terrestrial ecosystems. In order to reveal the tendency of soil quality of wolfberry (Lycium barbarum L.) orchard, make the origin of L. barbarum as the objective region, different stand ages of soil were selected in wolfberry orchard. The objective of the study was achieved by nematode and phospholipid fatty acid (PLFA) biomarker analysis of soil samples from L. barbarum orchards in the objective region. The change rule of soil nematode and microbial community diversity with the change of season, soil layer and stand age was analyzed. The results showed that the abundance of nematode was increased and then decreased in 0~20cm of soil, with the highest nematode at the stand age of 6. The proportion of bacterivores was the highest (57.23% and 61.19% in summer and autumn, respectively), and plant parasites nematode was next, fungivorous nematode and predatorsomnivore had the lowest abundance. Plant parasites nematode was relatively higher at 20~40cm than that at 0~20cm. The average concentrations of total and bacterial PLFAs in the surface soil were initially decreased and then increased, and the highest microbial PLFA concentrations were obtained in 9th year. The tendency change of total and bacterial PLFAs were similar to nematode abundance at 20~40cm in summer. With longer stand age, Shannon diversity index (H′) and richness index (SR) of nematode were increased and then decreased, however, H′ and SR of microbial were decreased, and dominant index was increased. The change tendency of abundance, H′ and SR of soil nematode were similar to soil microbial. There was significant negative correlation between soil pH value, EC, microbial and bacteria PLFA. The organic matter, total nitrogen and available P were significantly positively correlated to the abundance of nematodes and concentration of microbial PLFA, respectively. Total abundance of nematode, bacterivores and bacteria, fungi and actinomyces PLFA were significantly positively correlated. On the whole, the season, layer and stand age had different effects on the nematode and microbial community, and the stand age had the least effect; the season, soil layer and 〖JP3〗stand age had more significant effect on microbe than nematode. Furthermore, the microenvironment of soil was improved and then declined gradually as the stand age increased in the same season as well as the soil layer.

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張俊華,張翼,李明.寧夏枸杞園土壤線蟲和微生物群落多樣性研究[J].農(nóng)業(yè)機(jī)械學(xué)報,2016,47(9):161-170. Zhang Junhua, Zhang Yi, Li Ming. Soil Nematode and Microbial Community Diversity in Lycium barbarum L. Orchard[J]. Transactions of the Chinese Society for Agricultural Machinery,2016,47(9):161-170.

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  • 收稿日期:2016-03-22
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  • 在線發(fā)布日期: 2016-09-10
  • 出版日期: 2016-09-10
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