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Water and fertilizer are the two important material resources in agricultural production, which are closely related to the yield and quality of crop. In order to discuss the effects of water and fertilizer coupling on growth, yield and quality of grape under drip irrigation with film mulching, the experiment of fertilization under drip irrigation with film mulching was conducted in plastic greenhouse. There were four irrigation levels with irrigation depth of 180mm (W1), 270mm (W2), 360mm (W3) and 450mm (W4), respectively; and four fertilization levels, including N 150kg/hm2+ P2O5 120kg/hm2+ K2O 165kg/hm2 (F1), N 225kg/hm2+ P2O5 180kg/hm2+ K2O 248kg/hm2 (F2), N 300kg/hm2+ P2O5 240kg/hm2+ K2O 330kg/hm2 (F3) and N 450kg/hm2+ P2O5 360kg/hm2+ K2O 495kg/hm2 (F4). A total of 16 treatments were designed. The experimental results showed that the new shoots of grape plant under the treatment F2 with W2 grew better among the treatments, and the chlorophyll content in grape leaves was at a high level in the whole growth period, which was 3.28mg/g, 3.77mg/g,3.65mg/g and 3.53mg/g at new shoots growth, anthesis, fruit enlargement and fruit coloring stages, respectively. The fruit shape index of treatment F3 coupled with W4 was the highest among all treatments; however, the differences between each other groups were not significant except treatments F1W1 and F1W2. In addition, the analysis of fruit yield and quality indicated that the grape fruit yield of treatment F2W2 was not the highest, but there was no significant difference between the yield of treatments F2W2 and F2W3, which got the highest yield. Besides, the fruit quality of treatment F2W2 was more perfect compared with the other treatments. The soluble solids content of fruit of treatment F2W2 was the highest among all treatments, which reached 19.64%. The soluble sugar content and hardness index of fruit of treatment F2W2 also showed advantage, which were 17.00% and0.71kg/cm2, respectively. Comprehensive analysis results showed that the fertilizer application rate of treatment F2 coupled with irrigation depth of treatment W2 had a positive significance on increasing yield and improving quality of grape fruit.