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釀醋廢水水解酸化流出物培養(yǎng)小球藻實驗研究
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江蘇省自然科學基金資助項目(BK20140540)、中國博士后科學基金資助項目(2014M551519、2015T80502),、中國科學院可再生能源重點實驗室開放基金資助項目(y507k11001)和江蘇大學高級人才基金資助項目(14JDG024,、15JDG061)


Cultivation of Chlorella sp. Using Hydrolyzate of Vinegar Fermentation Wastewater
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    摘要:

    釀醋廢水經(jīng)水解酸化預處理后,,在柱式光生物反應器中利用不同體積分數(shù)的水解酸化液流出物進行了小球藻培養(yǎng)實驗,。研究表明釀醋廢水經(jīng)水解酸化處理后COD、氨氮,、總磷,、揮發(fā)性脂肪酸均有不同程度的減少,其中COD,、總磷含量分別減少了61.8%,、63.6%,,揮發(fā)性脂肪酸成分變化幅度較大,。當釀醋廢水水解酸化流出物體積分數(shù)高于40%,小球藻生長明顯受到抑制,,10%~30%體積分數(shù)的水解酸化流出物培養(yǎng)的小球藻生長較好,,藻細胞濃度可達6.6×107個/mL,,并且流出物添加磷酸鹽后,小球藻在低體積分數(shù)廢水流出物生長沒有延滯現(xiàn)象,。培養(yǎng)7d后,,小球藻對釀醋廢水水解酸化流出物的凈化效果顯著,氨氮,、總磷幾乎全部去除,,添磷條件下廢水流出物COD降低達96.6%。小球藻在體積分數(shù)為30%的水解酸化流出物生長含油量最高為24%,,C16-C18的脂肪酸含量在83.0%~95.5%之間,,該小球藻具備一定的生物柴油開發(fā)潛力。

    Abstract:

    After the hydrolysis acidification of vinegar fermentation wastewater, the cultivation of Chlorella sp. and nutrients removal in different concentration hydrolyzates of vinegar fermentation wastewater were investigated in the vertical tubular photobioreactor. Results showed that after the hydrolysis acidification of vinegar fermentation wastewater, the removal rates of COD, ammonia, volatile fatty acids (VFAs) and total phosphorus (TP) were all decreased to some extent. The removal rates of COD and TP were 61.8% and 63.6%, respectively. The components of VFAs were changed significantly. When the volume fraction of hydrolyzate was more than 40%, the growth rate of Chlorella sp. was inhibited. When the volume fraction of hydrolyzate was between 10% and 30%, the growth rate of Chlorella sp. was good. The concentration of Chlorella sp. cell could achieve 6.6×107cells/mL. The growth curves of Chlorella sp. were no lag phase, after the addition of phosphate in the hydrolyzate of vinegar fermentation wastewater. After 7d cultivation, the ammonia and total phosphorus were almost completely removed. The 96.6% COD removal was achieved after addition of some phosphate. The oil content (24%) was observed using 30% volume fraction hydrolyzate of vinegar fermentation wastewater. The contents of fatty acids C16-C18 were between 83.0% and 95.5%, which had good potential for the development for biodiesel utilization.

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霍書豪,樊 迪,徐 玲,王志武,王忠銘,黃達明.釀醋廢水水解酸化流出物培養(yǎng)小球藻實驗研究[J].農(nóng)業(yè)機械學報,2015,46(9):198-205. Huo Shuhao, Fan Di, Xu Ling, Wang Zhiwu, Wang Zhongming, Huang Daming. Cultivation of Chlorella sp. Using Hydrolyzate of Vinegar Fermentation Wastewater[J]. Transactions of the Chinese Society for Agricultural Machinery,2015,46(9):198-205.

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  • 收稿日期:2015-07-11
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  • 在線發(fā)布日期: 2015-09-10
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