0.88);蒙特卡羅模擬結果表明,,忽略氣單胞菌在食物接觸表面的存活行為會高估其在廚房內的交叉污染水平,。"/>

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氣單胞菌在不同食物接觸表面的存活與交叉污染
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國家自然科學基金面上資助項目(31271896)、上海市自然科學基金面上資助項目(12ZR1420500)和上海市研究生創(chuàng)新基金資助項目(10-14-308-101)


Survival and Cross-contamination of Aeromonas spp. on Different Food Contacts
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    摘要:

    為探討氣單胞菌在不同食物接觸表面的存活狀況,,采用擦拭取樣法測定氣單胞菌于案板,、刀具表面的恢復率,并將氣單胞菌分別接種于潔凈和被污染的案板,、刀具表面,,評定氣單胞菌在不同介質表面、不同時間下的存活狀況,。根據(jù)恢復率和介質表面上氣單胞菌存活實測值,,采用@Risk軟件對介質表面實時帶菌量的期望值進行計算,并運用一級線性模型對氣單胞菌存活特征曲線進行擬合,。同時,,以案板為交叉污染途徑,對氣單胞菌在介質表面的存活行為對交叉污染的影響進行了探討,。結果表明:采用擦拭取樣法測得的氣單胞菌從案板,、刀具表面的恢復率的最佳分布擬合結果分別是Normal(0.66,0.15)和Normal (0.72,,0.18),;氣單胞菌在被污染的介質表面存活的時間(7h)長于其在潔凈介質表面的存活時間(3h或2.5h),且一級線性模型可以較好地對氣單胞菌存活曲線進行擬合(R2>0.88),;蒙特卡羅模擬結果表明,,忽略氣單胞菌在食物接觸表面的存活行為會高估其在廚房內的交叉污染水平。

    Abstract:

    The aim of present work is to study the cross contamination and survival capacity of Aeromonas spp. on different food contacts in the kitchen. The recovery ratios of Aeromonas spp. using swabbing method from cutting boards and knives were firstly examined. Then, the survival of Aeromonas spp. on clean and contaminated cutting boards, stainless steel knives surfaces was studied. Based on the survival data and recovery ratios of Aeromonas spp., expected numbers of Aeromonas spp. on cutting boards and knives were calculated. Mathematical models describing survival of Aeromonas spp. as a function of time were also proposed. Meanwhile, in term of cutting boards as the cross contamination approach, combining survival model and further study were conducted to investigate the influence of the survival of Aeromonas spp. on the cross contamination level. Results indicated that the best fitting of recovery ratios of Aeromonas spp. from cutting boards and knives obtained by @Risk were Normal (0.66, 0.15) and Normal (0.72, 0.18), respectively. And Aeromonas spp. proved to survive longer on the contaminated cutting boards and knives than on the clean ones. Survival data for Aeromonas spp. could be well described by linear model (R2>0.88), and models without survival models included would overestimate the risk associated to bacterial transfer.

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董慶利,王海梅,Pradeep K Malakar,陸冉冉.氣單胞菌在不同食物接觸表面的存活與交叉污染[J].農業(yè)機械學報,2015,46(3):228-234. Dong Qingli, Wang Haimei, Pradeep K Malakar, Lu Ranran. Survival and Cross-contamination of Aeromonas spp. on Different Food Contacts[J]. Transactions of the Chinese Society for Agricultural Machinery,2015,46(3):228-234.

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  • 收稿日期:2014-10-31
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  • 在線發(fā)布日期: 2015-03-10
  • 出版日期: 2015-03-10
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