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基于微流控芯片的圓褐固氮菌濃度快速檢測方法
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江蘇省自然科學基金資助項目（BK20131250）和江蘇省高校優(yōu)勢學科建設工程資助項目(PAPD)(蘇政辦發(fā)（2011）6號)


Rapid Detection of Azotobacter chroococcum Concentration Based on Microfluidic Chip
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    摘要:

    針對當前微生物肥料中圓褐固氮菌濃度檢測方法耗時長、自動化程度低等缺點,，提出了一種基于微流控芯片的圓褐固氮菌濃度檢測方法,。設計了具有自動進樣,、混合稀釋以及電化學循環(huán)伏安檢測等功能的微流控芯片,，構建了微流控檢測系統(tǒng)實驗平臺，優(yōu)化了系統(tǒng)中的進樣速度,、掃描速率等工藝參數(shù),，并對檢測系統(tǒng)的性能指標進行了實驗驗證。結果表明,，與國標平板計數(shù)法相比,，所提出方法不僅能夠滿足微生物肥料檢測的精度要求，而且具備較快的檢測速度及較高的檢測自動化程度,。

    Abstract:

    Aiming at the problem that the concentration detection of Azotobacter chroococcum in microbial fertilizer suffers from time consuming and low automation level, a detecting method of Azotobacter chroococcum concentration based on microfluidic chip was proposed．A dedicated microfluidic chip with automatic sample injecting, dilution and cyclic voltammetry detection was designed, and the experiment platform based on microfluidic detection system was bulit. The optimum scan rate and feed rate of concentration detection in the microfluidic detection system were determined, and the performance index of the system was experimentally verified. Experiment results showed that, compared with plate counting method, the proposed method met the requirement of microbial fertilizer detection and realized rapid detection with increased automation.

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郭建江,張榮標,楊 寧,徐佩鋒.基于微流控芯片的圓褐固氮菌濃度快速檢測方法[J].農業(yè)機械學報,2015,46(1):155-159. Guo Jianjiang, Zhang Rongbiao, Yang Ning, Xu Peifeng. Rapid Detection of Azotobacter chroococcum Concentration Based on Microfluidic Chip[J]. Transactions of the Chinese Society for Agricultural Machinery,2015,46(1):155-159.

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  • 收稿日期:2014-03-06
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  • 在線發(fā)布日期: 2015-01-10
  • 出版日期: 2015-01-10
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