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The high intensity pulsed electric fields (PEF) technique was applied for assisted extraction of mussel polysaccharides to improve the efficiency of mussel polysaccharides extraction. The single factor test and Box Behnken design results showed that electrical field strength and pulse number were the significant affecting factors on the extraction efficiency of mussel polysaccharides. With mathematical regression model analysis of extraction efficiency and the impact factor, the optimal combination of parameters was found that electrical field strength was 25kV/cm, pulse number was 8, water powder ratio was 40mL/g. Under the optimized condition, the extraction rate of 2g mussel was up to 4.99% (polysaccharides wet weight sample ratio). Compared with ultrasonic technique, high intensity pulse electric fields is less time consuming with higher extraction efficiency. The study can provide a reference for further studies of extraction of mussel polysaccharides.