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穩(wěn)態(tài)化米糠蛋白超聲波輔助提取及其性質研究
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“十二五”國家科技支撐計劃資助項目(2012BAD34B02),、黑龍江省教育廳科學技術研究重點項目(12511z006)和哈爾濱市學科帶頭人基金資助項目(2012RFXXN107)


Study on Ultrasound-assisted Extraction and Properties of Rice Bran Protein
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    摘要:

    以穩(wěn)態(tài)化米糠為原料,研究超聲波-堿溶酸沉聯(lián)用技術提取米糠蛋白。以超聲波處理時間、超聲波功率、超聲波處理溫度及液料比為考察因素,,以米糠清蛋白和谷蛋白提取率為目標值進行單因素試驗,并對超聲波最佳條件下獲得米糠清蛋白和谷蛋白氨基酸組成、微觀結構,、二級結構及熱穩(wěn)定性進行分析研究。結果表明:超聲波處理時間10min,、超聲波功率160W,、超聲波處理溫度50℃、液料比10mL/g條件下,,米糠清蛋白,、谷蛋白提取率均可達最大值,;氨基酸組成、微觀結構及二級結構分別表明穩(wěn)態(tài)化米糠清蛋白和谷蛋白仍具有其典型特征,,且熱穩(wěn)定性較高,。

    Abstract:

    The rice bran protein was extracted using stabilized rice bran by ultrasonic combined with acid alkali soluble. Selecting the ultrasonic time, ultrasonic power, ultrasonic temperature and solid liquid ratio as studying factors, the yeild of rice bran albumin and rice bran glutelin were chosen for single factor experiment. Rice bran albumin and rice bran glutelin were from stabilized rice bran under the best ultrasonic conditions ,which were tested amino acid composition, microstructure, secondary structure and thermal stability. The results shows that the yeild of rice bran albumin and rice bran glutelin both reach the maximal value under the condition of ultrasonic time 10min, ultrasonic power 160W, ultrasonic temperature 50℃ and liquid-solid ratio 10mL/g. Amino acid composition, microstructure and secondary structure showed the two protein had the typical characteristics of albumin and protein, and had higher thermal stability.

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劉海飛,肖志剛,王 娜,王 鵬,馬 濤.穩(wěn)態(tài)化米糠蛋白超聲波輔助提取及其性質研究[J].農(nóng)業(yè)機械學報,2014,45(11):235-241. Liu Haifei, Xiao Zhigang, Wang Na, Wang Peng, Ma Tao. Study on Ultrasound-assisted Extraction and Properties of Rice Bran Protein[J]. Transactions of the Chinese Society for Agricultural Machinery,2014,45(11):235-241.

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  • 收稿日期:2013-11-18
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  • 在線發(fā)布日期: 2014-11-10
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