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An experimental study was carried on a Chinese V diesel engine fueled with Chinese V diesel blended with waste cooking oil biodiesel. The correlation between the characteristics of biodiesel and regulated emissions was investigated. The result shows that when fueled with blended fuel, higher oxygen content of the blended fuel led to higher NOx emission, but the increased extent was not obvious. NOx emission of all fuel was highly correlated with high temperature combustion field and R2 of their logarithmic function fitting curve was more than 0.97. Higher oxygen content of blend fuels led to lower THC and CO emissions.THC and CO emissions were highly correlated with combustion duration and R2 of their power function fitting curve was more than 0.96. The total particle number and nuclei mode particle number were higher, and the accumulation mode particle number was lower.Higher nuclei mode particle number was related to higher viscosity and sulfur content, and lower accumulation mode particle number was related to higher oxygen content.