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It is important to construct virtual plant model based on the interaction between plant and environment for greenhouse environment control. Taking the greenhouse cucumber growth as an example, it would obtain plant environment interaction information with data pre-processing and association analysis method. According to the crop development theory model, the information response model as well as information feedback model could be established. Moreover, the models could be coupled with the plant development process. The plant topological model and organ morphological model would be founded using regularization method and object-oriented technology, and the virtual plant model could be constructed. The experimental verification results indicated that the goodness of fit of models could be up to 95%, and improved that the presented method was effective in simulating the plant development under the interaction of environment, which also provided valuable evidences for dynamical control and prediction of greenhouse environment conditions fitting to plant growth.