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銅綠假單胞菌的溫度、pH值和乳酸鈉主參數(shù)模型構(gòu)建
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國(guó)家自然科學(xué)基金資助項(xiàng)目(31271896)和上海市自然科學(xué)基金資助項(xiàng)目(12ZR1420500)


Modelling Establishment of the Cardinal Parameters Model as a Function of Temperature, pH Value and Sodium Lactate for Pseudomonas aeruginosa
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    摘要:

    采用全自動(dòng)微生物生長(zhǎng)曲線分析儀Bioscreen測(cè)定分離自腐敗冷卻豬肉的銅綠假單胞菌在不同溫度(25~40℃)、pH值(5.0~7.5),、乳酸鈉質(zhì)量濃度(0~0.035g/mL)影響下的生長(zhǎng)曲線。基于最小二乘法運(yùn)用Matlab軟件中的fminsearch函數(shù)擬合銅綠假單胞菌遲滯期關(guān)于溫度,、pH值和乳酸鈉質(zhì)量濃度的主參數(shù)模型,經(jīng)參數(shù)估計(jì)求得銅綠假單胞菌的最低生長(zhǎng)溫度,、最適生長(zhǎng)溫度,、最高生長(zhǎng)溫度、最低生長(zhǎng)pH值,、最適生長(zhǎng)pH值,、最高生長(zhǎng)pH值、乳酸鈉的最小抑菌濃度,,進(jìn)而通過(guò)10組隨機(jī)數(shù)據(jù)進(jìn)行驗(yàn)證,。結(jié)果表明,構(gòu)建的主參數(shù)模型能較好預(yù)測(cè)銅綠假單胞菌的相關(guān)生長(zhǎng)參數(shù),,決定系數(shù)R2為0.9291,,偏差因子Bf為1.0975,準(zhǔn)確因子Af為1.3936,,平方根誤差為1.5989,。驗(yàn)證組的決定系數(shù)R2為0.8546,偏差因子Bf為1.1225,,準(zhǔn)確因子Af為1.2117,,都在可接受的范圍內(nèi)。

    Abstract:

    An automated turbidimetric system, Bioscreen C, was used to monitor the growth of Pseudomonas aeruginosa, which was separated from spoilage pork at a range of temperature (25~40℃), pH (5.0~7.5) and mass concentration of sodium lactate (0~0.035g/mL). The cardinal parameters model (CPM) was built on the basis of nonlinear least squares method using fminsearch function of Matlab software, and CPM represented the lag time of P.aeruginosa growth as a function of temperature, pH value and sodium lactate mass concentration. The CPM is able to determine the cardinal parameters Tmin, Topt, Tmax, Pmin, Popt, Pmax and the minimum inhibitory concentration of sodium lactate, respectively. This model was validated with ten random data within above ranges. The results showed that the growth parameters are predicted well by CPM with R2=0.9291, Bf=1.0975, Af=1.3936, and E= 1.5989, respectively. The validation parameters are 0.8546, 1.1225 and 1.2117 for R2, Bf and Af, respectively.

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董慶利,姚遠(yuǎn),趙勇,陳浩.銅綠假單胞菌的溫度,、pH值和乳酸鈉主參數(shù)模型構(gòu)建[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2014,45(1):197-202. Dong Qingli, Yao Yuan, Zhao Yong, Chen Hao. Modelling Establishment of the Cardinal Parameters Model as a Function of Temperature, pH Value and Sodium Lactate for Pseudomonas aeruginosa[J]. Transactions of the Chinese Society for Agricultural Machinery,2014,45(1):197-202.

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  • 收稿日期:2013-06-30
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  • 在線發(fā)布日期: 2014-01-03
  • 出版日期: 2014-01-03
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