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產(chǎn)香酵母分離鑒定與蘋果酒發(fā)酵中的應(yīng)用
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“十二五”國家科技支撐計(jì)劃資助項(xiàng)目(2012BAD31B01),、國家自然科學(xué)基金資助項(xiàng)目(31071550)和農(nóng)業(yè)部“948”資助項(xiàng)目(2011-G8-3)


Isolation and Identification of Aroma-producing Yeasts and Its Application in Cider Fermentation
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    摘要:

    為獲得可用于蘋果酒發(fā)酵的產(chǎn)香酵母,,提高蘋果酒的香氣質(zhì)量,采集蘋果果庫空氣樣和蘋果樣品,經(jīng)分離純化、產(chǎn)酯試驗(yàn)篩選得到6株具有產(chǎn)香能力的酵母,,對其進(jìn)行形態(tài)觀察及相關(guān)生理生化特性研究,,對ITS rDNA序列進(jìn)行擴(kuò)增和克隆測序,,結(jié)合與標(biāo)準(zhǔn)菌株的ITS同源性分析和系統(tǒng)發(fā)育樹的構(gòu)建,,明確了6株分離菌株的遺傳學(xué)位置,其中菌株YN6被鑒定為Wickerhamomyces anomalus,,該菌株產(chǎn)香能力較強(qiáng),,且能提高蘋果酒的感官質(zhì)量。蘋果酒的GC-MS分析結(jié)果表明,,與對照菌株WLS21獨(dú)立發(fā)酵結(jié)果相比,,接種YN6混合發(fā)酵后,主要揮發(fā)性香氣物質(zhì)的質(zhì)量濃度由248.09 mg/L 提高到496.14 mg/L,。

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    Air samples were taken from an apple granary in order to find aroma-producing yeasts for cider fermentation. After performing separation, purification and ester production tests, six yeasts were obtained, which showed ester producing abilities. The colony morphology, cell morphology, liquid cultural characteristics, physiological and biochemical characteristics and ITS complete sequences of the six tested yeasts were analyzed. The six yeast stains were identified by sequence homology comparison with known sequences in GenBank and by constructing phylogenetic tree. Among the six yeasts, the strain YN6 identified as Wickerhamomyces anomalus, had the strongest aroma-producing ability,,and could improve the sensory quality of cider. The results of GC-MS showed that, the cider the amounts of volatile compounds in the cider fermented by control cider yeast WLS21 was 248.09mg/L. But when mixed with YN6, the volatile compounds increased to 496.14mg/L.

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葉萌祺,袁亞宏,岳田利,甄幀.產(chǎn)香酵母分離鑒定與蘋果酒發(fā)酵中的應(yīng)用[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2013,44(12):187-192. Ye Mengqi, Yuan Yahong, Yue Tianli, Zhen Zhen. Isolation and Identification of Aroma-producing Yeasts and Its Application in Cider Fermentation[J]. Transactions of the Chinese Society for Agricultural Machinery,2013,44(12):187-192.

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  • 在線發(fā)布日期: 2013-12-05
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