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A virtual test system used to measure angular velocity on the top of driver seat was constructed based on CAN and TCP/IP bus. The program running in PC was designed based on LabVIEW, of which the status machine structure ensured the program to be easily read and maintained, and the queue structure increased efficiency of program implementing data sampling and processing in parallel. The system had the characteristics of high degree of integration, small size of sensors, convenient installation and function expansion on the spot. Four ride comfort regulations at home and abroad were comparatively analyzed and the test system constructed was used and verified in road way tests. The influence of angular velocity on the top of driver seat on evaluation index increased with the vehicle velocity, and it neared 20% (ISO standard) when the vehicle velocity reached 70km/h so that it could not be ignored. Additionally, the estimation method of acceleration PSD (power spectral density) and sampling frequency of original signal had a major impact on the evaluation index. Relatively speaking, the time domain method was more stable and reliable. However, it was difficult to determine the filter accorded with frequency domain weighting.