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According to the phototactic choice, sensitive recognition and phototactic gain stimulated by flashing and alternating light of locust, the regulatory illumination of array stripe light source was used to conduct regulatory illuminance experiment. The different spectral illumination combinations were compared. The mechanism of locust’s phototactic effect was discussed. The results suggested that combined mode of spectral illumination of array stripe and regulatory illumination could enhance locust’s phototactic visual gain, regulate locust’s phototactic optokinetic effect, stimulate locust’s phototactic aggregation effect, and increase locust’s phototactic activity. Locust’s phototactic effect was decided by spectral illumination energy and regulatory illuminance mode of array stripe. The optimum stimulation mode was consisted of 30ms luminous interval of frequency violet light array and 640ms luminous cycle of alternating green-violet-blue vertical stripe light array. The results of locusts stimulated by regulatory illumination of light source in the breeding shed showed that locusts’ phototactic effect was better at 20：00~23：00. When it continue to 24：00, the total response and aggregation rate were 80%±5% and 50%±5%, respectively. The locust’s biological habits and alertness, locust’s phototactic inertia, environmental factors and LED restriction restricted locust’s phototactic effect.