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Effect of treatment with 1-MCP or its structural analogues on ethylene synthesis and senescence of ‘Hanfu’ apple during cold storage was investigated. After being fumigated for 20 h with 0.75 μL/L 1-MCP (1-methylcylopropene), 2μL/L 1-PentCP (1-pentylcyclopropene) or 0.5μL/L 1-OCP (1-octylcyclopropene) respectively, the apple fruits were packed with 0.02 mm thick plastic bags and then stored at (0±0.5)℃. The results showed that treatment with 1-MCP or its structural analogues reduced ethylene production and respiration rate, delayed the respiration and ethylene peak by 30 d compared with control fruits. The content of ACC(1-aminocyclopropane 1-carboxylic acid) in treated fruits exhibited higher level than untreated control fruits throughout the whole cold storage, because treatment with 1-MCP or its structural analogues promoted the activity of ACS (ACC synthetase). All the treatments inhibited the activity of ACO (ACC oxidase), therefore, ethylene production of the treated fruits was suppressed during the cold storage. In addition, 1-MCP and its structural analogue also deferred the increase in MDA content and membrane permeability, decreased respiration rate and ethylene production, and delayed senescence of ‘Hanfu’ apple. However, there existed no significant differences between treatments with 1-MCP or its structural analogues. 