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The microbial growth kinetics model at constant temperature was built up. The effect of variable temperature on microbial growth state was found. Then the recorded variable temperatures were processed. Microbial growth kinetics model combined with Baranyi model under variable temperatures was obtained. A simulation experiment was also carried out to validate the accuracy of model. The determination coefficient was 0.956, the root mean square error was 0.165, the deviation factor was 0.994, and the accuracy factor was 1.134. The results showed that the model was adaptive to predict the growth of microbiology in the actual cold chain logistics.