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陜西省果庫中嗜酸耐熱菌的分離鑒定
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“十二五”國家科技支撐計(jì)劃資助項(xiàng)目(2012BAD31B01); 國家自然科學(xué)基金面上項(xiàng)目(31071550,、31171721);農(nóng)業(yè)部“948”項(xiàng)目


Isolation and Identification of Alicyclobacillus from Shaanxi Apple Libraries
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    摘要:

    為探明陜西省果庫中嗜酸耐熱菌的分布情況,,采用酸化的AAM培養(yǎng)基對(duì)陜西果庫中的空氣和蘋果中的嗜酸耐熱菌進(jìn)行分離純化,,與參比菌株A.acidoterrestris DSM3922T進(jìn)行細(xì)胞形態(tài)、菌落形態(tài)和生長(zhǎng)條件,、生理生化特征的比較分析,,并以16S rDNA序列分析構(gòu)建系統(tǒng)發(fā)育樹,,明確了8株分離菌的遺傳學(xué)位置,。分離株LC-4與標(biāo)準(zhǔn)菌的形態(tài)和生理生化特性有很大的相似性,16S rDNA序列分析表明LC-4與標(biāo)準(zhǔn)菌的同源性達(dá)99%,,可判定為酸土脂環(huán)酸芽孢桿菌,,其他7株屬于芽孢桿菌。結(jié)果表明嗜酸耐熱菌在果庫中確有分布,,可能對(duì)果汁質(zhì)量造成威脅,,需要加強(qiáng)控制。

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    In order to make sure the distribution of Alicyclobacillus from apple libraries in Shaanxi province, the Alicyclobacillus in apple and air were isolated and purified by using acidified AAM medium. The cell morphology, colony morphology, growing conditions, cultural characteristics and physiological characteristics were compared with the reference strain A.acidoterrestris DSM3922T. A phylogenetic tree based on 16S rDNA sequence analysis was constructed. The genetic location of eight isolated strains was clear. The isolated strain LC-4 was basically consistent with the reference stain Alicyclobacillus acidoterrestris DSM3922 T in morphology and physiological characteristics. By 16S rDNA sequence analysis, isolated strain LC-4 shared a homology of 99% with the reference strain, so it belonged to A.acidoterrestris and the other seven strains belonged to Bacillus ginsengihuimi. The results suggested Alicyclobacillus was distributed in orchard soil, which might pose a threat to the quality of fruit juice, and needed to be controlled.

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岳田利,耿玉麗,袁亞宏,郭彩霞.陜西省果庫中嗜酸耐熱菌的分離鑒定[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2013,44(7):187-193. Yue Tianli, Geng Yuli, Yuan Yahong, Guo Caixia. Isolation and Identification of Alicyclobacillus from Shaanxi Apple Libraries[J]. Transactions of the Chinese Society for Agricultural Machinery,2013,44(7):187-193.

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  • 在線發(fā)布日期: 2013-06-20
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