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The removal of CO2 from a CO2/CH4 mixture using commercial carbon-based adsorbents was researched at ambient temperature (25℃) under low pressure (0.2MPa). The performance in separating CO2 from its mixture with CH4 using carbon-based adsorbents was compared with 13X molecular sieve. The results indicated carbon molecular sieve could separate the mixture well but its selectivity was lower than 13X molecular sieve. However, carbon molecular sieve could be well regenerated, which was much better than 13X molecular sieve. These results suggested that carbon-based molecular sieve might be more suitable than zeolites for CO2 removal from biogas in a pressure swing adsorption cycle.