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The effect of ultra high pressure (UHP) treatment on conformation of S-ovalbumin was studied with Raman spectra and fluorescence spectra analysis. The effect of UHP on some functional properties of S-ovalbumin, such as solubility, emulsibility and foamability, were also investigated. It was showed that no significant changed in the molecular weight distribution of S-ovalbumin after UHP. Along with the increase of pressure, the content of random coil of S-ovalbumin was increasing gradually, the stability decreasing owing to the conformation change of disulfide bonds, and tyrosine was buried in the side chain, accordingly the compactly plate sheet structure was to become loose. The fluorescence intensity showed a slight increase after UHP under 100MPa, but then a sharp decline with the increase of pressure, no shift of the fluorescence emission peak happening, so it was pointed out that UHP induced conformational change. The solubility, emulsibility and foamability properties of S-ovalbumin had been improved significantly with the increase of pressure, and achieved the best effect at 300MPa. Then, there was a drop down when the pressure was 500MPa.