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Taking the 11% glutamic acid fermented liquid as testing samples, the effects of vacuum membrane distillation technology on the VMD of glutamic acid, decolorization and reuse of waste water were explored. The effects of heating temperature and concentration multiple on VMD enrich were also examined. The research results indicated that in the VMD process, with the membrane distillation technology, the glutamic acid concentrate in the glutamic acid filtrate liquid and the decolorization liquid was increased from 11% to 63.2% and 11% to 65.17%, respectively. Decolorizing process by using active carbon was able to reduce membrane fouling and improve membrane flux. After L9(34) orthogonal decolorizing test, variance analysis result showed that active carbon was the priority factor to decolorization. The level of activated carbon was 30g/L. The decolorization time was 0.5h and the revolving speed was 120r/min. The results of water product on membrane distillation and GC testing indicated that the peak time of seven volatile substances in water product were similar to the glutamic acid filtrate and they were inferred the same materials. Therefore, the water product by filtrate membrane distillation can be reused to original fermentation liquid and improve water utilization efficiency.