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According to locust’s response characteristic stimulated by light, sound and vibration, the experiment of locust’s phototactic response stimulated by light-sound-vibration coupling effect was carried out by using the designed experimental equipment. The results showed that light sound vibration coupling stimulated locust’s phototactic effect. The stimulation effect of its pull-push and drive could realize locust’s phototactic induction effectively. The illumination of combined spectrum decided locust’s phototactic aggregation. The illumination increased by 10 times, the effect of locust’s phototactic aggregation stimulated by light-sound-vibration coupling increased by 25%. Within a certain range, light-sound-vibration stimulated locust’s phototactic direction response effectively. The regulative spectrum illumination leaded to locust’s phototactic response. Vibration decided the driving stimulation of locust’s phototactic response. The audio frequency played the important role in gain motivation. Light-sound-vibration signal stimulated locust’s multiple receptors and generated the superimposition effect contributing to locust’s phototactic aggregation, so locust’s phototactic induction effect could be enhanced effectively through combining different audio frequency mode, regulatory mode of spectral illumination and circulating vibration mode.