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基于阻抗法的洛菲不動(dòng)桿菌生長(zhǎng)模型
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“十一五” 國(guó)家科技支撐計(jì)劃資助項(xiàng)目（2009BADB9B06）


Growth Model of Acinetobacter lwoffi Based on Impedance Method
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    摘要:

    利用平板計(jì)數(shù)法和阻抗法研究環(huán)境因子（溫度和pH值）對(duì)洛菲不動(dòng)桿菌最大生長(zhǎng)速率比,、培養(yǎng)液電導(dǎo)、導(dǎo)納最大增長(zhǎng)速率比的影響,，采用二次旋轉(zhuǎn)組合設(shè)計(jì),，分別獲得了洛菲不動(dòng)桿菌最大生長(zhǎng)速率比對(duì)數(shù)值,、電導(dǎo)最大增長(zhǎng)速率比對(duì)數(shù)值、導(dǎo)納最大增長(zhǎng)速率比對(duì)數(shù)值與溫度、pH值關(guān)系的多項(xiàng)式方程（Pr<0.05）,，并對(duì)建立的方程進(jìn)行了驗(yàn)證。結(jié)果表明,，洛菲不動(dòng)桿菌最大生長(zhǎng)速率模型優(yōu)于另兩個(gè)模型,，R2=0.9455，另兩個(gè)模型在最大增長(zhǎng)速率比小于0.06時(shí)效果較好,。分別將阻抗法獲得的電導(dǎo)最大增長(zhǎng)速率比,、導(dǎo)納最大增長(zhǎng)速率比與平板計(jì)數(shù)法獲得的洛菲不動(dòng)桿菌最大生長(zhǎng)速率比進(jìn)行相關(guān)關(guān)系分析，建立了立方模型,，模型的R2分別為0.859和0.806,。

    Abstract:

    Acinetobacter lwoffi was studied by impedance and plate count method throughout rotational combination design to define the effects of temperature and pH value on maximal specific growth rate (μm) of A. lwoffi, conductance and admittance. Polynomial models were established (Pr<0.05) and validated. The results showed that the established model with viable count method was better than the other two (R2=0.9455), which fit well when μm was less than 0.06. The correlation equation between μm of conductance/admittance and μm of A. lwoffi were established and the R2 were 0.859 and 0.806, respectively.

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劉東紅,唐佳妮,徐淑臻,張愛萍.基于阻抗法的洛菲不動(dòng)桿菌生長(zhǎng)模型[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2012,43(7):130-135,123. Liu Donghong, Tang Jiani, Xu Shuzhen, Zhang Aiping. Growth Model of Acinetobacter lwoffi Based on Impedance Method[J]. Transactions of the Chinese Society for Agricultural Machinery,2012,43(7):130-135,123.

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  • 在線發(fā)布日期: 2012-07-02
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