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UV-C照射后番茄差異表達(dá)基因的基因芯片檢測
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國家自然科學(xué)基金資助項(xiàng)目（30972036）


Microarray Analysis of Gene Expression Profile Induced by Postharvest UV-C Irradiation in Tomato Fruit
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    摘要:

    應(yīng)用Affymetrix基因芯片研究了4kJ/m2短波紫外線（UV-C）照射后7d番茄總基因表達(dá)譜的變化情況。結(jié)果表明，UV-C照射后7d，5177個(gè)基因在雜交后發(fā)生變化，差異表達(dá)變化倍數(shù)值在大于2和小于-2范圍內(nèi)基因共481個(gè)，其中上調(diào)基因331個(gè)，下調(diào)基因150個(gè)。上調(diào)基因主要包括細(xì)胞壁相關(guān)基因、信號轉(zhuǎn)導(dǎo)基因、脅迫相關(guān)基因和抗性基因等；下調(diào)基因主要包括乙烯相關(guān)基因和初級代謝基因等。研究結(jié)果為探索采后UV-C照射誘導(dǎo)番茄抗性反應(yīng)、抑制乙烯合成，從而推遲成熟、延長番茄采后壽命的分子機(jī)制提供了基礎(chǔ)。

    Abstract:

    To obtain an overall view on transcript modification during the tomato fruit in response to postharvest UV-C irradiation (4kJ/m2), a microarrary analysis was performed by using Affymetrix tomato genechip arrays. The results showed that 331 and 150 genes were up- or down-regulated, respectively, more than two folds in postharvest UV-C irradiated tomato fruit as compared with that in control fruit. The up-regulated genes mainly involved cell wall related, signal transduction, stress related and defense response. Conversely, genes related to ethylene and primary metabolism were generally down-regulated. These results opened ways to probe into the molecular mechanisms of the effects of postharvest UV-C irradiation on increasing disease resistance, delaying ripening and prolonging postharvest life in tomato fruit.

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劉長虹,蔡路昀,韓曉旭,應(yīng)鐵進(jìn). UV-C照射后番茄差異表達(dá)基因的基因芯片檢測[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2012,43(1):134-140. Liu Changhong, Cai Luyun, Han Xiaoxu, Ying Tiejin. Microarray Analysis of Gene Expression Profile Induced by Postharvest UV-C Irradiation in Tomato Fruit[J]. Transactions of the Chinese Society for Agricultural Machinery,2012,43(1):134-140.

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  • 在線發(fā)布日期: 2012-01-12
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