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山核桃外果皮黃酮提取與純化
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安徽省高校省級自然科學(xué)基金重點(diǎn)資助項(xiàng)目（KJ2010A293、2006KJ190B）


Extraction and Purification of Flavone in C.cathayensis Exocarp
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    摘要:

    采用有機(jī)溶劑萃取法提取山核桃外果皮黃酮化合物,，通過單因素和正交試驗(yàn)確定了最佳工藝參數(shù)。結(jié)果表明，提取溶劑為體積分?jǐn)?shù)70%乙醇,、提取時(shí)間4h,、提取溫度60℃,、液料比15mL/g條件下得黃酮化合物最高提取率為1.14%。采用硅膠色譜柱層析法純化黃酮化合物,，在液固比3mL/g的條件下，體積分?jǐn)?shù)70%乙醇以2mL/min的流速進(jìn)行洗脫,，黃酮純度提高了34.6%,，總黃酮回收率達(dá)89.46%。

    Abstract:

    In order to study the extraction and purification of flavonein C.cathayensis exocarp, organic solvent extraction was used to extrat flavone compound in C.cathayensis exocarp. Through single fraction experiments and orthogonal experiments, the optimal technological parameters were determined. The result showed the maximum extraction ration of flavone compound was 1.14% under the condition of 70% ethanol, time of 4h, temperature of 60℃, liquidsolid ratio of 15mL/g. Silica gel chromatography was used to purify falconoid, liquidsolid ratio of 3mL/g, gradient eluted by 70% ethanol at the speed of 2mL/min, the purity of flavone was improved by 34.6%, and its recovery reached to 89.46%. 

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陳向明,徐濤,查甫本.山核桃外果皮黃酮提取與純化[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2011,42(12):177-181. Chen Xiangming,Xu Tao,Zha Fuben. Extraction and Purification of Flavone in C. cathayensis Exocarp[J]. Transactions of the Chinese Society for Agricultural Machinery,2011,42(12):177-181.

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  • 在線發(fā)布日期: 2011-12-19
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