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基于酶?jìng)鞲衅鞯霓r(nóng)藥濃度便攜式實(shí)時(shí)測(cè)量裝置
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國(guó)家高技術(shù)研究發(fā)展計(jì)劃（863計(jì)劃）資助項(xiàng)目（2008AA100905、2006AA10A305-3）;江蘇省屬高校自然科學(xué)重大基礎(chǔ)研究項(xiàng)目(10KJA470007)


Portable and Real-time Device for Measuring Concentration of Pesticides Used with Enzyme Sensing Electrode
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    摘要:

    設(shè)計(jì)了一種應(yīng)用酶?jìng)鞲衅鞯谋銛y式農(nóng)藥濃度測(cè)量裝置,，由絲網(wǎng)印刷酶電極、信號(hào)調(diào)理電路和單片機(jī)C8051F040組成,。酶?jìng)鞲须姌O修飾方法使涂層電荷轉(zhuǎn)移電阻減小而加速電荷轉(zhuǎn)移。裝置檢測(cè)出酶抑制率與農(nóng)藥（西維因）濃度的對(duì)數(shù)在農(nóng)藥（西維因）質(zhì)量濃度為5ng/mL~2μg/mL時(shí)呈線性關(guān)系,，最低檢出限為1.7ng/mL,，檢出時(shí)間小于161s，系統(tǒng)尺寸小,，可采用電池供電,，為數(shù)量級(jí)μg/L的農(nóng)藥質(zhì)量濃度現(xiàn)場(chǎng)檢測(cè)(如農(nóng)藥噴霧現(xiàn)場(chǎng))提供了一種便攜式快速測(cè)量裝置。

    Abstract:

    A kind of portable device for measuring concentration of pesticides used with enzyme sensor was designed. It included screen-printed enzyme electrode, signal conditioning circuit and MCU C8051F040. The preparation of enzyme electrode made the coated resistance of charge transfer reduced, charge transfer accelerated; and a reliable liner relationship between the inhibition of enzyme and log ［carbaryl］ was obtained from 5ng/mL to 2μg/mL, with the detection limitation of 1.7ng/mL. Response time of the device was less than 161s. The size of the device was small and the power was supported by battery. It provided a portable device for rapid measuring and spotty concentration of pesticide in μg/L level.

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陳志剛,張啟甲,邱白晶,王坤,吳春篤.基于酶?jìng)鞲衅鞯霓r(nóng)藥濃度便攜式實(shí)時(shí)測(cè)量裝置[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2011,42(11):178-182. Chen Zhigang, Zhang Qijia, Qiu Baijing, Wang Kun, Wu Chundu. Portable and Real-time Device for Measuring Concentration of Pesticides Used with Enzyme Sensing Electrode[J]. Transactions of the Chinese Society for Agricultural Machinery,2011,42(11):178-182.

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